BM5015 Adeno-Associated Virus 2 (VP1/VP2/VP3) antibody
see related secondary antibodiessee all 8 Adeno-Associated Virus 2 products
50 µg / US$ 555
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Adeno-Associated Virus 2 (VP1/VP2/VP3) B1
Synonyms
AAV2,
Product Description
Mouse anti Adeno-Associated Virus 2 (VP1/VP2/VP3) B1, Presentation: Purified. Product is tested for Frozen sections ( C ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | B1 |
| Catalog Number | BM5015 |
| Quantity | 50 µg |
| Price | US$ 555 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM5015.pdf |
Datasheet Extract
| Immunogen | Adeno-associated virus capsid proteins and virus particles |
| Format | State: Lyophilised Purification: Protein A affinity purified BufferSystem: Contains 0.09% sodium azide as preservative and 0.5% BSA as stabilizer Reconstitution: Reconstitute with 1 ml distilled water |
| Applications | Immunofluorescence microscopy.
Immunoprecipitation (mainly free VP proteins). Immunohistochemistry on frozen sections (Fixation: Acetone or formaldehyde; Dilute 1:10 with PBS or Tris-buffered saline; Incubation time: 1h at RT). Also useful for Western Blot or Affinity chromatography. |
| Specificity | Useful for immunolocalization studies of capsid formation in combination with monoclonal antibody A20 (Cat. No. BM5010). B1 reacts with free VP 1, VP 2 and VP 3 of adeno-associated virus and at a very reduced degree with assembled capsids. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope mapping experiments (Wobus et al., see below) identified aa726 to aa733 (C-terminus; common to all 3 VP proteins) as the specific binding region. The antibody is also useful for characterization of different stages of infection.
Reacts with AAV-2, found in human and monkey, and all published AAV serotypes (AAV-4 only weak cross-reaction). Species: Human, monkey. |
| Storage | Store the antibody at 2-8°C after reconstitution. Shelf life: One year from despatch. |
| References | Wistuba A.,Weger S., Kern A., and Kleinschmidt J. (1995) Intermediates of adeno-associated virus type 2 assembly: Identification of soluble complexes containing Rep and Cap proteins. J. Virol. 69, 5311-5319.
Wistuba A., Kern A., Weger S., Grimm D., and Kleinschmidt J. (1997) Subcellular compartmentalization of adeno-associated virus type 2 assembly. J. Virol. 71, 1341-1352. Wobus C.A., Hügle-Dörr B., Girod A., Petersen G., Hallek M. and Kleinschmidt J.A. (2000) Monoclonal antibodies against the adenoassociated virus type 2 (AAV-2) capsid: Epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virology 74, 9281-9293. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 315 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 305 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 325 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 465 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'BM5015 Adeno-Associated Virus 2 (VP1/VP2/VP3)'.
