BM5086P Synaptopodin antibody
see related secondary antibodiessee all 3 Synaptopodin products
50 µg / US$ 515
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Synaptopodin G1D4
Product Description
Mouse anti Synaptopodin G1D4, Presentation: Purified. Product is tested for Frozen sections ( C ), Immunofluorescence ( IF ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Immunofluorescence ( IF ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB ) |
| Reactivity | Bovine ( Bov ), Genua Pig ( GP ), Gerbil ( Gerbil ), Human ( Hu ), Mouse ( Ms ), Rat ( Rt ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | G1D4 |
| Catalog Number | BM5086P |
| Quantity | 50 µg |
| Price | US$ 515 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM5086P.pdf |
Datasheet Extract
| Background | Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data); in mouse the corresponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (the difference might be attributed to posttranslational modifications). |
| Immunogen | Isolated rat kidney glomeruli |
| Format | State: Lyophilized Ig fraction Purification: Protein A affinity chromatography BufferSystem: Final solution contains 0.09 % NaN3, 0.5 % BSA in PBS buffer, pH 7.4 Reconstitution: Restore with 1 ml dist. water. |
| Applications | Immunohistochemistry on frozen sections and paraffin embedded tissue after microwave treatment: 1/5-1/10, 1 h at RT.
Western blotting (the antibody also reacts with a 44 kD degradation fragment of synaptopodin). Immunofluorescence. |
| Specificity | The antibody reacts specifically with synaptopodin. The antibody recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with a-actinin. Does not react with parietal cells.
It reacts with a subset of exclusively telencephalic synapses. Differentiation dependent expression during postnatal maturation of rat brain. Differentiation dependent expression in cultured hippocampal neurons. Species: Human, bovine, rat, mouse, guinea pig, gerbil. Negative with rabbit, frog and chicken. |
| Storage | Store the antibody at 2-8°C (lyophilized) and after reconstitution in aliquots at -20°C.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Mundel P, Gilbert P, and W Kriz. Podocytes in Glomerulus of Rat Kidney Express a Characteristic 44 kD Protein. J Histochem and Cytochem Vol 39 No 8:1047-1056 (1991)
2. Mundel P and W Kritz. Structure and function of podocytes: an update. Anat Embryol 192:385-397 (1995) 3. Mundel P, Reiser J, Kriz W. Phenotypic conversion and differentiation of human and rat podocytes in vitro. J Am Soc Nephrol 8:8978-705 (1997) 4. Mundel P, Heid HW, Mundel TM, Krüger M, Reiser J, Kriz W. Synaptopodin, an actin-associated protein in telencephalic dendrites and in renal podocytes. J Cell Biol Vol 139 (1):193-204 (1997) 5. Mundel P, Reiser J, Zuniga Borja A, Davidson G, Pavenstädt H, Kriz W, Zeller R. Rearrangements of cytoskeleton and cell contacts induce process formation and postmitotic differentiation of conditionally immortalized mouse podocyte cell lines, Exp Cell Res 236: 248-258 (1997) 6. Kobayashi N, Kriz W, Kuriyma R, Mundel P: Non-uniform microtubular polarity, established by CHO1/MKLP1 motor protein, is necessary for process formation of podocytes. J Cell Biol 143: 1961-1970 (1998) 7. Barisoni L, Kriz W, Mundel P, D'Agati V. The dysregulated podocyte phenotype: a novel concept in the pathogenesis of collapsing idiopathic focal segmental glomerulosclerosis and HIV-associated nephropathy. J Am Soc Nephrol 10: 51-61 (1999) 8. Kihara I, Yaoita E, Kawasaki K, Yamamoto T, Hara M, Yanagihara T: Origin of hyperplastic epithelial cells in idiopathic collapsing glomerulopathy. Histopathology 34(6): 537-547 (1999) 9. Asanuma K, Kim K, Oh J, Giardino L, Chabanis S, Faul C, Reiser J, Mundel P: synaptopodin regulates the actin-bundling activity of α-actinin in an isoform-specific manner. J Clin Invest 115, 1188-1198 (2005). |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 315 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 305 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 325 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 465 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'BM5086P Synaptopodin'.