BM5516PX M13 Bacteriophage Coat Protein g8p antibody
see related secondary antibodiessee all 7 M13 Bacteriophage Coat Protein g8p products
0.5 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti M13 Bacteriophage Coat Protein g8p B62-FE2
Product Description
Mouse anti M13 Bacteriophage Coat Protein g8p B62-FE2, Presentation: Purified. Product is tested for Enzyme Immunoassay ( E ), Westernblot / Immunoblot ( WB )
Properties
| Applications | Enzyme Immunoassay ( E ), Westernblot / Immunoblot ( WB ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG2b |
| Clone | B62-FE2 |
| Catalog Number | BM5516PX |
| Quantity | 0.5 mg |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM5516PX.pdf |
Datasheet Extract
| Monoclonal Antibody to M13, fd, F1 Filamentous Phages | |
| Concentration | 5.0 mg/ml |
| Immunogen | fd phages from E. coli F+ strain (JM109) |
| Format | State: Lyophilized purified IgG Purification: Protein A affinity chromatography BufferSystem: 10 mM Tris-HCl buffer, pH 8.0, 300 mM NaCl For prolonged storage of reconstituted antibody solution we strongly recommend addition of a stabilizing protein (e.g. 0.5% BSA) and a preservative (e.g. thimerosal or sodium azide) Reconstitution: Restore in 0.1 ml dist. water |
| Applications | Phage display: immunoassay for identification of recombinant antigen- or antibody-phages; Detection limit: 107 phage particles.
Immunoblotting. Immunoelectron microscopy. ELISA: 1:50 000. |
| Specificity | B62-FE2 binds to an epitope on pVIII (phage coat protein) covering the N-terminal region of g8p AEGDDPAKAAFDSLQASAT (cf. Kneissel et al.) |
| Add. Information | Antibody shows a tendency to precipitate at neutral pH. |
| Storage | Prior to reconstitution store at 2-8°C.
Following reconstitution store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Micheel B, Heymann S, Scharte G, Böttger V, Vogel V, Dübel S, Breitling F, Little M, Behrsing O: Production of monoclonal antibodies against epitopes of the main coat protein of filamentous fd phages. J. Immunol. Methods 171, 103-109 (1994).
2. Kneissel S, Queitsch I, Petersen G, Behrsing O, Micheel B, Dübel S. Epitope structures recognised by antibodies against the major coat protein (g8p) of filamentous bacteriophage fd (Inoviridae). J. Mol. Biol. 288, 21-28 (1999) 3. Rondot S, Koch J, Breitling F, Dübel S: A helper phage to improve single-chain antibody presentation in phage display. Nature Biotechnology 19, 75-78 (2001). |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / 180,00 € | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / 160,00 € | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / 160,00 € | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / 170,00 € | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / 190,00 € | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / 200,00 € | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / 320,00 € | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / 320,00 € | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / 320,00 € | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / 320,00 € | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / 320,00 € | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / 330,00 € | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / 330,00 € | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 330,00 € | |||||
Click here to see all secondary antibodies for 'BM5516PX M13 Bacteriophage Coat Protein g8p'.
