BP4524 Aurora-B / STK12 / ARK2 antibody
see related secondary antibodiessee all 14 Aurora-B / STK12 / ARK2 products
0.2 ml / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Sheep anti Aurora-B / STK12 / ARK2 -
Synonyms
Aurora kinase B Aurora B STK-1 AIK2 AIM1 AURKB Serine/Threonine-Protein Kinase 12, Aurora- and Ipl1-like Midbody-Associated Protein 1, Aurora/IPL1-Related Kinase 2
Product review
Please read our product review about Aurora-B / STK12 / ARK2: Antibody Panel for the Detection of Aurora Kinase Family.
Product Description
Sheep anti Aurora-B / STK12 / ARK2 -, Presentation: Aff - Purified. Product is tested for Westernblot / Immunoblot ( WB )
Properties
| Applications | Westernblot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Aff - Purified |
| Host | Sheep |
| Catalog Number | BP4524 |
| Swiss Prot. No. | Q96GD4 |
| Quantity | 0.2 ml |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BP4524.pdf |
Datasheet Extract
| Polyclonal Antibody to Aurora B | |
| Alternate names | AIK2, AurB; AURKB, Aurora-B, IPL1
|
| Background | Aurora B is a mitotic protein kinase that phosphorylates histone H3 (probably on Serine 10), behaves as a chromosomal passenger protein, and may regulate several stages of mitosis such as centrosome separation, chromosome segregation and cytokinesis. It localizes to the inner centromere region from prophase to anaphase. |
| Immunogen | Recombinant human His(6)-Aurora B [residues 1-344] expressed in insect cells. Swiss Num.: Q96GD4 |
| Format | State: Liquid purified Ig fraction. Purification: Affinity chromatography.. BufferSystem: PBS with 0.09% sodium azide as preservative. |
| Applications | Western blot: 0.2 µg/ml. |
| Specificity | Recognises human Aurora B. |
| Storage | Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| Protocols | Western Blotting Protocol:
1. Transfer gel to PDVF or nitrocellulose membrane 2. Place membrane in plastic tray in blocking buffer for one hour with agitation 3. Rinse in wash buffer 4. Incubate in wash buffer plus primary antibody for one hour 5. Wash 6 X 5 minutes with wash buffer 6. Incubate in wash buffer plus secondary antibody for one hour 7. Wash 6X 5 minutes with wash buffer 8. Detect (e.g. ECL, Amersham according to manufacturers instructions) Wash buffer: PBS + 0.1% Tween 20 Blocking buffer: Wash buffer + 5% dried milk powder The concentration of antibodies used depends on each antibody, the amount of antigen and the detection method used. Generally, dilution is in the range of a few hundred times dilution to a few thousand times dilution, but usually has to be determined empirically. |
| Pictures | Figure 1. Western blot of Anti-Aurora B on recombinant Auror.
|
7 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1456B | Sheep IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / 170,00 € | |||||
| R1456F | Sheep IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / 160,00 € | |||||
| R1456T | Sheep IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / 160,00 € | |||||
| R1456TR | Sheep IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / 170,00 € | |||||
| R1456HRP | Sheep IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / 190,00 € | |||||
| R1456AP | Sheep IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / 210,00 € | |||||
| R1433R | Sheep IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 330,00 € | |||||
Click here to see all secondary antibodies for 'BP4524 Aurora-B / STK12 / ARK2'.