BP5040 Bombesin antibody
see related secondary antibodiessee all 26 Bombesin products
0.25 ml / US$ 395
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Bombesin
Synonyms
Gastrin-releasing peptide, GRP, ,
Product review
Please read our product review about Bombesin: Antibodies to Gastrin-releasing Peptide (Bombesin) and its Receptors (BRS-3 ,GRP-R and Neuromedin B-Receptor).
Product Description
Rabbit anti Bombesin , Presentation: Serum. Product is tested for Frozen sections ( C ), Immunofluorescence ( IF ), Paraffin Sections ( P )
Properties
| Applications | Frozen sections ( C ), Immunofluorescence ( IF ), Paraffin Sections ( P ) |
| Reactivity | Amphibian ( Amph ), Human ( Hu ), Mouse ( Ms ), Rat ( Rt ) |
| Presentation | Serum |
| Host | Rabbit |
| Catalog Number | BP5040 |
| Quantity | 0.25 ml |
| Price | US$ 395 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BP5040.pdf |
Datasheet Extract
| Immunogen | Synthetic frog bombesin conjugated to bovine thyroglobulin |
| Format | State: Liquid serum |
| Applications | Immunofluorescence: 1/400 - 1/800 for IF microscopy using FITC with overnight incubation at 2-8°C.
Immunohistochemistry on frozen sections and paraffin embedded tissues (proteolytic treatment recommended - see protocol). Recommended positive control: Gut (peripheral nerves), fetal lung. Working dilution: 1:50 for immunohistochemistry, dilute immediately before use. Dilution buffer: PBS with 1% albumin and 0.1% Na-Azide. Incubation time: 30 min at room temperature or overnight at 2-8°C. |
| Specificity | This polyclonal antibody can be used for the study of endocrine differentiation in tumors of the respiratory tract. Species: Human, frog, rat and mouse. |
| Storage | Store the antibody undiluted at 2-8°C for up to one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. |
| Protocols | Protocol for the use of anti-Bombesin polyclonal antibody on paraffin-embedded tissue sections
FIXATION: Small tissue blocks are fixed in 4% buffered formaldehyde (pH 7.6) at 4°C. The fixation time should not exceed 12 h. During the embedding process temperatures should not exceed 58°C. STAINING PROCEDURE: Deparaffinize and rehydrate sections. If necessary, block endogeneous peroxidase activity (0.3% H2O2 in 50% methanol, 15 min). Rinse in PBS 3x. Incubate for 5 min in 0.1% pronase in PBS at 37°C. Rinse in PBS 3x. Apply properly diluted anti-Bombesin antibody (1:50) and incubate for 30 min at room temperature or overnight at 4°C. Rinse 3x. Apply conjugated antibody. Rinse in PBS 3x. Apply substrate. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'BP5040 Bombesin'.
