CL030 CD49D / ITGA4 antibody
see related secondary antibodiessee all 92 CD49D / ITGA4 products
0.5 ml / US$ 385
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rat anti CD49D / ITGA4 R1-2
Synonyms
ITGA4, Integrin alpha-IV, VLA-4, CD49 antigen-like family member D, CD49d, Integrin alpha-4,
Product Description
Rat anti CD49D / ITGA4 R1-2, Presentation: Ascites. Product is tested for Frozen sections ( C ), Flow cytometry ( F ), Immunoprecipitation ( IP )
Properties
| Applications | Frozen sections ( C ), Flow cytometry ( F ), Immunoprecipitation ( IP ) |
| Reactivity | Mouse ( Ms ) |
| Presentation | Ascites |
| Host | Rat |
| Isotype | IgG2b |
| Clone | R1-2 |
| Catalog Number | CL030 |
| Swiss Prot. No. | Q00651 |
| Quantity | 0.5 ml |
| Price | US$ 385 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  CL030.pdf |
Datasheet Extract
| Alternate names | Integrin alpha 4 chain |
| Background | alpha4 integrin combines with beta1 and beta7integrin to form VLA-4 and LPAM-1 (Peyers patch homing receptor) respectively. VLA-4 is expressed on most peripheral lymphocytes, thymocytes and monocytes. LPAM-1 is found on peripheral lymphocytes, but few thymocytes. Fibronectin and VCAM-1 act as ligands for both VLA-4 and LPAM-1. LPAM-1 also binds the mucosal vascular addressin MAdCAM-1. (1) |
| Immunogen | Peyers Patch HEV binding lymphoma line (TK1) Swiss Num.: Q00651 |
| Format | State: Lyophilized ascitic fluid Reconstitution: Restore with 0.5 ml of cold distilled water. |
| Applications | Flow cytometry (see protocol/specificity).
Immunoprecipitation. Immunohistochemistry. (1,2,3). |
| Specificity | CL030 reacts with a4 integrin, which helps to mediate cell-cell and cell-matrix interactions.
Tissue Distribution by Flow Cytometry Analysis (see protocols below): Rat Strain: BALB/c Cell Concentration : 1x10e6 cells per tests Antibody Concentration Used: 1:1000 in 50µl/10 cells Isotypic Control: Rat IgG2b Cell Source Percentage of cells stained above control (see protocols below): TK1 cells 99.3% Spleen 89.1% Thymus 92.1% Bone Marrow 67.4% Strain Distribution by Flow Cytometry Analysis (see protocols below): Tissue: Spleen Cell Concentration : 1x10e6 cells per tests Antibody Concentration Used: 1:500 in 50µl Strains Tested: BALB/c, C57BL/6, C3H/He, CBA/J, AKR Positive: BALB/c, C57BL/6, C3H/He, CBA/J, AKR Negative: none Species: Mouse. |
| Add. Information | Appropriate control samples should always be included in any labelling studies. |
| Storage | Store lyophilized product at 2 - 8 °C for up to one month or at -20°C for longer. Following reconstitution store in aliquots at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch. |
| References | 1) Berlin, C., E. L. Berg, M. J. Briskin, D. P. Andrew, P. J. Kilshaw, B. Holzmann, I. L. Weissman, A. Hamann, E.C. Butcher 1993. a4b7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCam-1. Cell 704:185-195.
2) Holzmann, B., I.. L. Weissman 1989. Peyer's patch-specific lymphocyte homing receptors consist of a VLA-4 like a chain associated with either of two integrin b chains, one of which is novel. EMBO 8:1736-1741. 3) Holzmann, B., B. W. McIntyre, I. W. Weissman 1989. Identification of a murine Peyer's patch-specific lymphocyte homing receptor as an integrin molecule with an a chain homologous to human VLA-4a. Cell 56:37-46. |
| Protocols | FLOW CYTOMETRY ANALYSIS:
Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 50ml of a 1:500-1000 dilution of antibody. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (FITC Goat anti-rat IgG (H+L)) at a 1/500 dilution. 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes are protected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C in media B. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls). |
| Pictures | Cell Source: TK1 Cells;
Percentage of cells stained above control: 99.3% |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1371B | Rat IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1371F | Rat IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1371T | Rat IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1371TR | Rat IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 305 | |||||
| R1371HRP | Rat IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 325 | |||||
| R1371AP | Rat IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 345 | |||||
| R1439C2 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1439C3 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1439C35 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1439C5 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1439C55 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1413R | Rat IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Ms, Rb, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
| R1428R | Rat IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
| R1415R | Rat IgG F(ab')2 F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'CL030 CD49D / ITGA4'.