CL030B CD49D / ITGA4 antibody
see related secondary antibodiessee all 92 CD49D / ITGA4 products
0.1 mg / US$ 325
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rat anti CD49D / ITGA4 R1-2
Synonyms
ITGA4, Integrin alpha-IV, VLA-4, CD49 antigen-like family member D, CD49d, Integrin alpha-4,
Product Description
Rat anti CD49D / ITGA4 R1-2, Presentation: Biotin. Product is tested for Frozen sections ( C ), Flow cytometry ( F )
Properties
| Applications | Frozen sections ( C ), Flow cytometry ( F ) |
| Reactivity | Mouse ( Ms ) |
| Presentation | Biotin |
| Host | Rat |
| Isotype | IgG2b |
| Clone | R1-2 |
| Catalog Number | CL030B |
| Swiss Prot. No. | Q00651 |
| Quantity | 0.1 mg |
| Price | US$ 325 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  CL030B.pdf |
Datasheet Extract
| Concentration | 0.1 mg/ml |
| Background | a4 integrin combines with b1 and b7integrin to form VLA-4 and LPAM-1 (Peyers patch homing receptor) respectively. VLA-4 is expressed on most peripheral lymphocytes, thymocytes and monocytes. LPAM-1 is found on peripheral lymphocytes, but few thymocytes. Fibronectin and VCAM-1 act as ligands for both VLA-4 and LPAM-1. LPAM-1 also binds the mucosal vascular addressin MAdCAM-1. |
| Immunogen | Peyers Patch HEV binding lymphoma line (TK1)
Donor: Fisher Spleen Fusion Partner: P3x63Ag8.653 Swiss Num.: Q00651 |
| Format | State: Liquid Purification: Protein G Chromatography BufferSystem: PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml Label: Biotin |
| Applications | Flow cytometry.
Immunoprecipitation. Immunohistochemistry. (1,2,3) |
| Specificity | This monoclonal antibody reacts with a4 integrin, which helps to mediate cell-cell and cell-matrix interactions. Species: Mouse. |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1) Berlin, C., E. L. Berg, M. J. Briskin, D. P. Andrew, P. J. Kilshaw, B. Holzmann, I. L. Weissman, A. Hamann, E.C. Butcher 1993. a4b7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCam-1. Cell 704:185-195
2) Holzmann, B., I.. L. Weissman 1989. Peyerâ??s patch-specific lymphocyte homing receptors consist of a VLA-4 like a chain associated with either of two integrin b chains, one of which is novel. EMBO 8:1736-1741 3) Holzmann, B., B. W. McIntyre, I. W. Weissman 1989. Identification of a murine Peyers patch-specific lymphocyte homing receptor as an integrin molecule with an a chain homologous to human VLA-4a. Cell 56:37-46 |
| Protocols | FLOW CYTOMETRY ANALYSIS:
Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add ~1.0 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (Streptavidin-FITC) at a 1:500 dilution. 9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Mouse Strain: BALB/c Cell Concentration: 1x10e6 cells per tests Antibody Concentration Used: 1.0 µg/10e6 cells Isotypic Control: Biotin Rat IgG2b Cell Source: Percentage of cells stained above control: TK1 Cells: 97.9% Thymus: 80.0% Spleen: 85.1% Bone Marrow: 72.4% |
| Pictures | |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1371B | Rat IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1371F | Rat IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1371T | Rat IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1371TR | Rat IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 305 | |||||
| R1371HRP | Rat IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 325 | |||||
| R1371AP | Rat IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 345 | |||||
| R1439C2 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1439C3 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1439C35 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1439C5 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1439C55 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1413R | Rat IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Ms, Rb, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
| R1428R | Rat IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
| R1415R | Rat IgG F(ab')2 F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'CL030B CD49D / ITGA4'.