CL037 CD90 / THY1 antibody
see related secondary antibodiessee all 88 CD90 / THY1 products
0.5 ml / US$ 385
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti CD90 / THY1 T11D7e
Synonyms
THY1, THY-1, CDw90, CD90, Thy-1 membrane glycoprotein,
Product Description
Mouse anti CD90 / THY1 T11D7e, Presentation: Ascites. Product is tested for Cytotoxicity Assay ( CT )
Properties
| Applications | Cytotoxicity Assay ( CT ) |
| Reactivity | Mouse ( Ms ), Rat ( Rt ) |
| Presentation | Ascites |
| Host | Mouse |
| Isotype | IgM |
| Clone | T11D7e |
| Catalog Number | CL037 |
| Swiss Prot. No. | P01831 |
| Quantity | 0.5 ml |
| Price | US$ 385 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  CL037.pdf |
Datasheet Extract
| Immunogen | Rat Thymocytes. Swiss Num.: P01831 |
| Format | State: Lyophilized (sterile filtered) ascites. Reconstitution: Restore with 0.5 ml of cold distilled water. |
| Applications | Functional assays. |
| Specificity | This antibody is specific for T cells from mouse strains expressing the Thy 1.1 phenotype (eg. AKR, RF).
It can be used to selectively identify or deplete T cells or their precursors. Cross reacts with rat Thy 1. |
| Storage | Store the lyophilized antibody at 2-8°C for one month or at -20°C for longer.
After reconstitution aliquot and store at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Protocols | CYTOTOXICITY ANALYSIS:
Method: 1. Prepare a cell suspension from the appropriate tissue in Cytotoxicity Medium A or equivalent. Remove red cells and dead cells (where necessary) by purification of viable lymphocytes on cell separation medium. After washing, adjust the cell concentration to 1x10e6 cells per ml in Cytotoxicity Medium. 2. Add the antibody to a final concentration of 1:80 and mix. 3. Incubate for 60 minutes at 4°C. 4. Centrifuge to pellet the cells and discard the supernatant. 5. Resuspend to the original volume in Rabbit Complementc diluted to the recommended concentration in Cytotoxicity Medium.. 6. Incubate for 60 minutes at 37°C. 7. Place on ice. 8. Add Trypan Blue, 10% by volume of 1% Trypan Blue (w/v) added 3-5 minutes before scoring works well. Score live versus dead cells in a hemacytometer. Results-Antibody Titration by Cytotoxicity Analysis: Cell Source: Thymus Donor: AKR/J Cell Concentration: 1.1x10e6 cells/ml Rabbit Complement (Concentration: 1:10). Tissue Distribution: Procedure: see before Antibody Concentration Used: 1/1280 Strain: AKR/J Cell Source-C.I. Thymus: 100 Spleen: 16 Lymph Node: 64 Bone Marrow: 9 Strain Distribution: Procedure: see before Antibody Concentration Used: 1:20 Strains Tested: AKR/J, C57BL/6, BALB/c, C3H/He Positive: AKR/J Negative: C57BL/6, BALB/c, C3H/He CYTOTOXICITY DEPLETION ASSAY: Method: 1. Prepare a cell suspension from the appropriate tissue in Cytotoxicity Mediuma. Remove red cells and dead cells (where necessary) by purification of viable lymphocytes on cell separation medium. After washing, adjust the cell concentration to 1x10e7 cells per ml in Cytotoxicity Medium. 2. Add the antibody to a final concentration of 1:80 and mix. Alternatively, pellet the cells and resuspend in antibody diluted 1:80 in Cytotoxicity Medium. 3. Incubate for 60 minutes at 4°C. 4. Centrifuge to pellet the cells and discard the supernatant. 5. Resuspend to the original volume in Rabbit Complementc diluted to the appropriate concentration in Cytotoxicity Medium. (Recommended concentration included with each batch of Rabbit Complement.) 6. Incubate for 60 minutes at 37°C. 7. Monitor for percent cytotoxicity at this stage, before further processing. For this purpose, remove a small sample from each tube, dilute 1:10 with medium, and add 1/10 volume of 1% Trypan Blue. After 3-5 minutes, score live versus dead cells in a hemacytometer. 8. For functional studies, remove the dead cells from the treated groups before further processing, particularly if the treated cells are to be cultured. This can be done by layering the cell suspension over a separation medium and centrifuging at room temperature as per the instructions provided. Live cells will form a layer at the interface, while the dead cells pellet. The interface can then be collected and washed in Cytotoxicity Medium before being resuspended in the appropriate medium for further processing. Alternatively, the cells can be washed and resuspended in the appropriate medium for further processing immediately after Step 6, provided that the dead cells will not interfere with subsequent assays. FUNCTIONAL TESTING: Method: Cells were treated as described in ''Cytotoxicity Depletion Assay''. Treated cells and controls were tested for: a) the ability to generate plaque-forming cells (PFC) using a modified Jerne haemolytic plaque assay. b) the ability to generate cytotoxic T effector cells using a cytotoxic lymphocyte reaction (CTL) assay. Cells were treated both before and after sensitization in the CTL assay. Results: Cell Source: Splenocytes Donors: AKR/J and BALB/c Cell Concentration: 1x10e7 cells/ml Antibody Concentration Used: 1:500 Complement: Rabbit Complement (Concentration Used: 1:10) Treatment of AKR/J splenocytes with this antibody plus complement resulted in a significant reduction in the number of plaque-forming cells. As assessed by a CTL assay, cytotoxic T cell function was essentially eliminated in both presensitized and postsensitized treated samples. No effect was observed when BALB/c cells were used. These results are consistent with the removal of T helper and T cytotoxic cell activity. |
| Pictures | |
10 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1349B | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 365 | |||||
| R1349F | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 345 | |||||
| R1349T | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 345 | |||||
| R1349TR | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 365 | |||||
| R1349HRP | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 375 | |||||
| R1349AP | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | AP | 1 mg / US$ 395 | |||||
| SP1018F | Mouse IgM mu chain | ||||||
| Goat | FITC | Ms | 1 mg / US$ 375 | ||||
| SP1018R | Mouse IgM mu chain | ||||||
| Goat | PE | Ms | 0.1 mg / US$ 485 | ||||
| SP1018HRP | Mouse IgM mu chain | ||||||
| Goat | HRP | Ms | 1 mg / US$ 375 | ||||
| SP1018AP | Mouse IgM mu chain | ||||||
| Goat | AP | Ms | 0.8 mg / US$ 425 | ||||
Click here to see all secondary antibodies for 'CL037 CD90 / THY1'.