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CL049B Mac-2 antibody
see related secondary antibodiessee all 2 Mac-2 products
0.1 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rat anti Mac-2 M3/38
Synonyms
Mac2
Product Description
Rat anti Mac-2 M3/38, Presentation: Biotin. Product is tested for Frozen sections ( C ), Enzyme Immunoassay ( E ), Flow cytometry ( F ), Paraffin Sections ( P )
Properties
| Applications | Frozen sections ( C ), Enzyme Immunoassay ( E ), Flow cytometry ( F ), Paraffin Sections ( P ) |
| Reactivity | Mouse ( Ms ) |
| Presentation | Biotin |
| Host | Rat |
| Isotype | IgG2a |
| Clone | M3/38 |
| Catalog Number | CL049B |
| Quantity | 0.1 mg |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  CL049B.pdf |
Datasheet Extract
| Monoclonal Antibody to MAC-2 - Biotin | |
| Concentration | 0.1 mg/ml |
| Immunogen | Plasma membrane glycoproteins from C57BL/6 mouse thioglycollateelicited peritoneal exudate |
| Format | State: Liquid BufferSystem: PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml Label: Biotin |
| Applications | CL049B can be used for indirect immunofluorescence staining, including flow cytometric analysis of live cells. The addition of propidium iodide is optional; its use eliminates staining artifacts caused by dead cells (1). This clone is also suitable for frozen sections (4,5) and ELISA (6). |
| Specificity | Anti-Mac-2 monoclonal antibody specifically binds the mouse Mac-2 antigen.The antibody recognizes a 32,000 Dalton surface antigen found on a subpopulation of mouse macrophages. It reacts with peritoneal exudate macrophages where the exudate is provoked by thioglycollate, protease peptone (20%), macrophages of lymphoid and non-lymphoid tissues, interdigitating dendritic cells and Langerhans cells. Mac-2 is also expressed in the cytoplasm on non-elicited resident macrophages; 5% are strongly reactive, the remaining 95% show much weaker staining.The antibody does not react with peritoneal exudate macrophages where the exudate is provoked by Listeria monocytogenes, lipopolysaccharide or concanavalin A. It also does not react with peritoneal macrophages, splenic macrophages, granulocytes, thymocytes, peripheral lymph node cells and with 99% of bone marrow cells. Species: Mouse. |
| Storage | Store at 2-8°C. For long term storage, aliquot and freeze unused portion at -20°C in volumes appropriate for single usage. Avoid freeze/thaw cycles. Shelf life: One year from despatch. |
| References | 1. Sasaki, K.T., Dumas, S.E. and Engleman, E.G. (1987) Cytometry 8:413.
2. Springer, T.A. (1981) J. Bio/. Chem. 256:3833. 3. Ho, M.K. and Springer, T.A. (1984) Methods in Enzymology 108:313. 4. Knisley, K.A. and Weitlauf, H.M. (1993) Journal of Reproduction and Fertility. 97:521 5. Weitlauf, H.M and Knisley, K.A. (1992) Biology of Repoduction. 46:811 6. Saada, A., Reichert, F. and Rotshenker, S. (1996) The Journal of Cell Biology. 133:159 |
| Protocols | FLOW CYTOMETRY ANALYSIS:
Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 2 µg* of CL049B per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (Streptavidin-FITC) at a dilution recommended by the manufacturer. 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes are protected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C in media B. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls). |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1371B | Rat IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / 170,00 € | |||||
| R1371F | Rat IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / 160,00 € | |||||
| R1371T | Rat IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / 160,00 € | |||||
| R1371TR | Rat IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / 170,00 € | |||||
| R1371HRP | Rat IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / 190,00 € | |||||
| R1371AP | Rat IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / 210,00 € | |||||
| R1439C2 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / 320,00 € | |||||
| R1439C3 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / 320,00 € | |||||
| R1439C35 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / 320,00 € | |||||
| R1439C5 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / 320,00 € | |||||
| R1439C55 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / 320,00 € | |||||
| R1413R | Rat IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Ms, Rb, Sh | ||||||
| Goat | PE | 1 ml / 330,00 € | |||||
| R1428R | Rat IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 330,00 € | |||||
| R1415R | Rat IgG F(ab')2 F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / 330,00 € | |||||
Click here to see all secondary antibodies for 'CL049B Mac-2'.
