CL049P Mac-2 antibody
see related secondary antibodiessee all 5 Mac-2 products
0.1 mg / US$ 285
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rat anti Mac-2 M3/38
Synonyms
Mac2,
Product Description
Rat anti Mac-2 M3/38, Presentation: Purified. Product is tested for Frozen sections ( C ), Enzyme Immunoassay ( E ), Flow cytometry ( F ), Immunofluorescence ( IF ), Paraffin Sections ( P )
Properties
| Applications | Frozen sections ( C ), Enzyme Immunoassay ( E ), Flow cytometry ( F ), Immunofluorescence ( IF ), Paraffin Sections ( P ) |
| Reactivity | Mouse ( Ms ) |
| Presentation | Purified |
| Host | Rat |
| Isotype | IgG2a |
| Clone | M3/38 |
| Catalog Number | CL049P |
| Quantity | 0.1 mg |
| Price | US$ 285 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  CL049P.pdf |
Datasheet Extract
| Monoclonal Antibody to Mouse MAC-2 | |
| Concentration | 1.0 mg/ml |
| Immunogen | Plasma membrane glycoproteins from C57BL/6 mouse thioglycollate-elicited peritoneal exudate |
| Format | State: Liquid purified immunoglobulin fraction BufferSystem: PBS with 0.02% sodium azide as preservative |
| Applications | Immunofluorescence staining, including flow cytometric analysis of live cells.
(The addition of propidium iodide is optional; its use eliminates staining artefacts caused by dead cells (1)). This antibody is also suitable for frozen sections (4,5), paraffin sections (7) and ELISA (6). |
| Specificity | Anti-Mac-2 monoclonal antibody specifically binds the mouse Mac-2 antigen.
The antibody recognizes a 32,000 dalton surface antigen found on a subpopulation of mouse macrophages. It reacts with peritoneal exudate macrophages where the exudate is provoked by thioglycollate, protease peptone (20%), macrophages of lymphoid and non-lymphoid tissues, interdigitating dendritic cells and Langerhans cells. Mac-2 is also expressed in the cytoplasm on non-elicited resident macrophages; 5% are strongly reactive, the remaining 95% show much weaker staining. The antibody does not react with peritoneal exudate macrophages where the exudate is provoked by Listeria monocytogenes, lipopolysaccharide, concanavalin A, peritoneal macrphages, spleenic macrophages, granulocytes, thymocytes, peripheral lymph node cells and with 99% of bone marrow cells. Results of Tissue Distribution by Flow Cytometry Analysis: - Mouse Strain: BALB/c - Cell concentration: 1 x 10e6 cells per test - Antibody concentration used: 0.5 μg/10e6 cells - Isotypic Control: Rat IgG2a Percentage of cells stained above control: Thymus = 1.41% Thioglycollate-elicited peritoneal exudate = 82% (see picture below) |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Sasaki, K.T., Dumas, S.E. and Engleman, E.G. (1987) Cytometry 8:413.
2. Springer, T.A. (1981) J. Bio/. Chem. 256:3833. 3. Ho, M.K. and Springer, T.A. (1984) Methods in Enzymology 108:313. 4. Knisley, K.A. and Weitlauf, H.M. (1993) Journal of Reproduction and Fertility. 97:521 5. Weitlauf, H.M and Knisley, K.A. (1992) Biology of Repoduction. 46:811 6. Saada, A., Reichert, F. and Rotshenker, S. (1996) The Journal of Cell Biology. 133:159 7. Kowala, M. C. et al. (2000) Atherosclerosis 149: 323-330 8. Cua, D.J. et al. (1995) Eur. J. Immunol. 25: 2318-2324 9. Ho, M-K. et al. (1982) J. of Immunol. 28(3):1221-1228 10. Mey, A. et al. (1996) J of Immunol. 156:1572-1577 11. Leenen, P.J.M. et al. (1986) Differentiation 32: 157-164 12. Huang, H. et al. (1992) Laboratory Investigation 67(1): 138-146 |
| Protocols | Flow Cytometry Analysis:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0.5 - 1.0 µg of antibody per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody FITC Goat anti-rat IgG (H+L) at appropriate dilution. 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes are protected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C in media B. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls). |
| Pictures | Cell source: Thioglycollate-elicited peritonperitoneal exudate. Percentage of cells stained above control: 82%
|
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1371B | Rat IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 295 | |||||
| R1371F | Rat IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1371T | Rat IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1371TR | Rat IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1371HRP | Rat IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1371AP | Rat IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1439C2 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1439C3 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1439C35 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1439C5 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1439C55 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1413R | Rat IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Ms, Rb, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1428R | Rat IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
| R1415R | Rat IgG F(ab')2 F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'CL049P Mac-2'.