CL083F T-Cell Receptor TCR Vb 11 antibody
see related secondary antibodiessee all 7 T-Cell Receptor TCR Vb 11 products
0.1 mg / US$ 325
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rat anti T-Cell Receptor TCR Vb 11 CTVB11
Synonyms
TCR Vb11, TCR V beta11,
Product Description
Rat anti T-Cell Receptor TCR Vb 11 CTVB11, Presentation: FITC. Product is tested for Flow cytometry ( F )
Properties
| Applications | Flow cytometry ( F ) |
| Reactivity | Mouse ( Ms ) |
| Presentation | FITC |
| Host | Rat |
| Isotype | IgG2b |
| Clone | CTVB11 |
| Catalog Number | CL083F |
| Quantity | 0.1 mg |
| Price | US$ 325 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  CL083F.pdf |
Datasheet Extract
| Concentration | 0.1 mg/ml |
| Immunogen | CBA/J |
| Format | State: Liquid purified Purification: Protein G Chromatography BufferSystem: PBS, 0.09% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. Label: FITC |
| Applications | Flow Cytometry. |
| Specificity | This monoclonal antibody reacts with the TCR Vb11 bearing T cells. TCR Vb11 may be deleted in mouse strains expressing the MHC class II I-E antigens. Species: Mouse. |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. This product is photosensitive and should protected from light. Shelf life: one year from despatch. |
| References | 1. Tomonari, K. and Lovering, E. 1989 T cell receptor-specific monoclonal antibodies against a Vb11-positive mouse T cell clone. Immunogentics 28:445-451.
2. Pircher, H., Ohashi, P., Miescher, G., Lang, R., Zikopoulos, A., Burki, K., Mak, T. W., MacDonald, H.R. and Hengartner, H. 1990 T cell receptor (TCR) b chain transgenic mice: studies on allelic exclusion and the TCR+ gamma/delta population. Eur. J. Immunol. 20:417-424. 3. Bill, J., Kanagawa, O., Woodland, D. and Palmer, E. 1989 The MHC molecule I-E is necessary but not sufficient for the clonal deletion of Vb11-bearing T cells. J. Exp. Med. 169:1405-1419. 4. Gao, E., Kanagawa, O. and Sprent, J. 1989 Capacity of unprimed CD4+ and CD8+ T cells expressing Vb11 receptors to respond to I-E alloantigens in vivio. J. Exp Med. 170: 1947-1957. |
| Protocols | FLOW CYTOMETRY ANALYSIS:
Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add ~1.0 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light, since most flurochromes are light sensitive.) 7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls). Results - Tissue Distribution by Flow Cytometry Analysis: Mouse Strain: C3H.SW Cell Concentration: 1x10e6 cells per tests Antibody Concentration Used: 1.0 µg/10e6 cells Isotypic Control: FITC Rat IgG2b |
| Pictures | Cell Source: Spleen - Percentage of cells stained above control: 1.01% |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1371B | Rat IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 295 | |||||
| R1371F | Rat IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1371T | Rat IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1371TR | Rat IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1371HRP | Rat IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1371AP | Rat IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1439C2 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1439C3 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1439C35 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1439C5 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1439C55 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1413R | Rat IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Ms, Rb, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1428R | Rat IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
| R1415R | Rat IgG F(ab')2 F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'CL083F T-Cell Receptor TCR Vb 11'.
