CL116A OX-43 Antigen antibody
see related secondary antibodiessee all 6 OX-43 Antigen products
1 mg / US$ 575
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti OX-43 Antigen OX-43
Synonyms
OX43,
Product Description
Mouse anti OX-43 Antigen OX-43, Presentation: Azide Free. Product is tested for Frozen sections ( C ), Flow cytometry ( F ), Immunoprecipitation ( IP )
Properties
| Applications | Frozen sections ( C ), Flow cytometry ( F ), Immunoprecipitation ( IP ) |
| Reactivity | Rat ( Rt ) |
| Presentation | Azide Free |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | OX-43 |
| Catalog Number | CL116A |
| Quantity | 1 mg |
| Price | US$ 575 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  CL116A.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml |
| Immunogen | Rat Peritoneal Macrophages
Immunocyte Donor: BALB/c Spleen Fusion Partner: NSO/U |
| Format | State: Liquid Purification: Protein G Chromatography BufferSystem: PBS, no preservative. |
| Applications | Flow Cytometry. |
| Specificity | This monoclonal antibody recognizes a surface protein of MW 90 kDa and generally reacts with all vascular endothelium in the rat except that of brain capillaries. This is the reciprocal tissue pattern to that of the transferrin receptor. It has been shown that the expression of this Ab is on the luminal surface of blood vessels. This antibody labels all peritoneal macrophages, a sub-population of alveolar macrophages (65%) and rare interstitial cells in the brain and heart. In addition, anti-rat endothelium monoclonal antibody labels circulating erythrocytes, 22% of peripheral blood mononuclear cells and 17% of nucleated cells in bone marrow. This antibody does not label granulocytes, dendritic cells, lymphocytes, or lymphocyte blasts, thymocytes, lymph node cells, mast cells and platelets. This antibody has been invaluable in the demonstration of molecular heterogeneity of vascular endothelium. Species: Rat. |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Handle under aseptic conditions. Shelf life: one year from despatch. |
| References | 1) Robinson, A.P., White, T.M. and D.W. Mason. (1985) Immunology. 57, 231-237. MRC OX-43: a monoclonal antibody which reacts with all vascular endothelium in the rat except that of brain capillaries. |
| Protocols | FLOW CYTOMETRY ANALYSIS:
Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-Rat cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0.5-1.0 µg* of this Ab. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 5 µl (1 µg) of secondary antibody (PE Goat anti-mouse F(ab1)2 IgG (H+L)) to each tube. 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes are protected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C in media B. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Rat Strain: Lewis Cell Concentration: 1x10e6 cells per test Antibody Concentration Used: 1.0 µg/10e6 cells Isotypic Control: Purified Mouse IgG1,k Cell Source Percentage of cells stained above control: Thymus:1.12% Peritoneal Macrophages:94.8% Results - Strain Distribution: Antibody Concentration: 1.0 µg/10e6 cells Strains Tested: Wistar, Brown Norway, Buffalo, Fischer 344 Positive: Buffalo, Brown Norway, Fischer 344, Wistar Negative: none |
| Pictures | |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 315 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 305 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 325 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 465 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'CL116A OX-43 Antigen'.