DA3504 Fibroblast growth factor 9 / FGF9 antigen
see all 18 Fibroblast growth factor 9 / FGF9 products
5 µg / US$ 235
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Human Fibroblast growth factor 9 / FGF9
Synonyms
FGF9, GAF, Fibroblast growth factor 9, FGF-9, HBGF-9, Glia-activating factor,
Product Description
Human Fibroblast growth factor 9 / FGF9
Properties
| Presentation | Purified |
| Species | Human |
| Purity | >95% |
| Source | Insect cells |
| Molecular Weight | 23 kD |
| Catalog Number | DA3504 |
| Swiss Prot. No. | P31371 |
| Quantity | 5 µg |
| Price | US$ 235 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  DA3504.pdf |
Datasheet Extract
| Background | The FGF family is composed of at least 22 polypeptides that show a variety of biological activities towards cells of mesenchymal, neuronal and epithelial origin. All members are heparin-binding growth factors. All FGFs have two conserved cysteine residues and share 30-50% sequence identity at the amino acid level. FGF-9, also named glia -activating factor, was originally identified and purified from the supernatant of a human glioma cell line as a mitogen for glial cells. FGF -9 has also been shown to stimulate the proliferation of oligodendrocytes type 2 astrocyte progenitor cells, PC 12 cells and Balb/c3T3 cells. Unlike acidic and basic FGF, the FGF-9 molecule is not a mitogen for human vascular endothelial cells. From the four FGF receptors known, FGF -9 binds only to the FGF-R3. Constitutive active FGF -R3 in human is connected with anormal bone development. The human FGF-9 cDNA encodes a 208 amino acid residue protein that contains a single, potential N-linked glycosylation site. The native protein is glycosylated and is efficiently secreted after synthesis, although FGF -9 lacks a typical secretion signal. Rat and mouse FGF-9 show a very high homology to human FGF-9. The transcripts for FGF-9 have been found in brain and in kidney tissue. |
| Immunogen | Swiss Num.: P31371 |
| Source | Insect cells |
| Format | State: Lyophilized without buffer stabilizer. Purity: > 95% by SDS-PAGE and visualised by silver stain. Endotoxinlevel: < 0.1 ng per µg of FGF-9. Reconstitution: It is recommended to add steril phosphate-buffered saline (or medium) containing at least 0.1% BSA/HSA (or FCS) to prepare a stock solution of no less than 100 ng/ml. For dilution it is recommended to use steril phasphate-buffered saline containing at least 0.1% BSA/HSA or using medium with FCS for dilution. |
| Description | Recombinant Human Human FGF-9. Biological Activity: The activity was estimated by stimulation of 3H-thymidine incorporation in quiescent Balb/c3T3 cells as desribed in Reference 1. The ED50 for this effect is 1.5-2.0 ng/ml. Maximal effect for DNA synthesis was observed at 5-7 ng/ml rhFGF-9 . Specific Activity: 6.6 x 10e5 units/mg Molecular weight: 23 kDa |
| Storage | Store lyophilized human FGF-9 at -20°C.
Reconstituted human FGF-9 should be stored at -20°C to -70°C. Avoid repeated freeze-thaw cycles! Shelf life: One year from despatch. |
| References | 1. Weich et al. 1990, Growth Factors, 2:313-320.
2. Miyamoto et al., 1993, Mol Cell Biol. 13:4251- 4259. 3. Hecht et al. 1995, Growth Factors 12:223-233. |