DA3505 Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) antigen
see all 64 Granulocyte Macrophage Colony Stimulating Factor products
2 µg / US$ 225
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)
Synonyms
GMCSF,
Product Description
Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)
Properties
| Presentation | Purified |
| Species | Human |
| Purity | >95% |
| Source | Insect cells |
| Molecular Weight | 14.6 kD |
| Catalog Number | DA3505 |
| Quantity | 2 µg |
| Price | US$ 225 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  DA3505.pdf |
Datasheet Extract
| Concentration | 5 x 10e4 units/vial |
| Background | Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), a 14,6 kDa protein consisting of 127 amino acid residues (Ala18-Glu144) and fused to a C-terminal His-tag (6x His), is a potent species specific stimulator of bone marrow cells and several other cell types.
GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages and eosinophils. GM-CSF has also been reported to have a functional role on non-hematopoietic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. GM-CSF is species specific and human GM-CSF has no biological effects on mouse cells. GM-CSF exerts its biological effects through binding to specific cell surface receptors. The high affinity receptors required for human GM-CSF signal transduction have been shown to be heterodimers consisting of a GM-CSF-specific α chain and a common β chain that is shared by the high-affinity receptors for IL-3 and IL-5. |
| Source | Insect cells |
| Format | State: Lyophilized without preservatives and stabilizers Purity: > 98 % by SDS-PAGE and visualised by silver stain Endotoxinlevel: < 0.1 ng per µg of GM-CSF Reconstitution: The lyophilized GM-CSF is soluble in water and most aqueous buffers. The lyophilized powder can be reconstituted in water to a concentration of 0.1 mg/ml. This solution can be diluted into other buffered solutions or stored at -20°C for future use. |
| Description | Recombinant human GM-CSF Biological Activity: Human GM-CSF is fully biologically active when compared to standards. The ED50 as determined by dose-dependent stimulation of the proliferation of human TF-1 cells is < / = 0.1 ng/ml. For most in vitro applications, this product exerts its biological activity in the concentration range of 0.05 to 1.0 ng/ml. Molecular weight: 14.6 kDa |
| Storage | The lyophilized powder though stable at room temperature, is best stored desiccated below 0°C. Reconstituted GM-CSF should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Shelf life: one year from despatch. |