DA3506X Hepatocyte Growth Factor (HGF) antigen
see all 19 Hepatocyte Growth Factor products
25 µg / US$ 515
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Human Hepatocyte Growth Factor (HGF)
Product Description
Human Hepatocyte Growth Factor (HGF)
Properties
| Presentation | Purified |
| Species | Human |
| Purity | >85% |
| Source | Insect cells |
| Molecular Weight | 78 kD |
| Catalog Number | DA3506X |
| Quantity | 25 µg |
| Price | US$ 515 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  DA3506X.pdf |
Datasheet Extract
| Background | Human Hepatocyte Growth Factor (HGF), also known as scatter factor, is a pleiotrophic cytokine that shows homology to the enzymes of the blood coagulation cascade. It stimulates the motility and invasion of several cancer cell types and can induce angiogenesis. Recently HGF was found to be identical to scatter factor, a fibroblast-derived factor promoting the dissociation of epithelial and vascular endothelial cell colonies in monolayer cell cultures by stimulating cell migration. HGF is synthesized as a biologically inactive single chain precursor, which is cleaved by a specific, extracellular serum serine protease to a fully active heterodimer. This mature, biologically active HGF consists of a disulfide-linked alpha-beta heterodimer of the two cleavage products. Previous studies have shown that single chain and heterodimeric HGF are equally active in in vitro assay systems due to either production of the serine protease in cell culture or the presence of the ubiquitious protease in serum. All biological responses induced by HGF are elicited by binding to its transmembrane tyrosine kinase receptor, which is encoded by the MET proto-oncogene. After autophosphorylation of the receptor different cytoplasmatic effectors are activated that bind to the same multifunctional docking site of the receptor. HGF function is essential for normal development. Knockout studies have demonstrated that both ligand and receptor deficient mice display an embryonic lethal phenotype. Hepatocytes have to be primed before they can fully respond to HGF. This priming requires cytokines as TNF and IL-6. Recent studies have suggested that HGF synergizes with basic FGF in the induction of angiogenesis.
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| Source | Insect cells |
| Format | State: Lyophilized without stabilizer or preservative Purity: > 90 % by SDS-PAGE and visualised by silver stain Endotoxinlevel: < 0.1 ng per µg of HGF Reconstitution: The lyophilized human HGF is soluble in water and most aqueous buffers. It can be restored in water to a concentration of 100 µg/ml. Further dilutions should be made into buffer containing protein or medium containing serum. |
| Description | Recombinant human HGF is a 78.0 kDa protein.
Biological Activity: Human HGF is fully biologically active when compared to standards. The activity was assayed for scattering activity in the MDCK cell assay. The ED50 was for this effect is typically at 0.5-1.0 ng/ml. |
| Storage | The lyophilized protein is stable at -20°C for one year from despatch.
Reconstituted human HGF is stable for three months when stored in working aliquots at -20°C. Avoid repeated freezing and thawing. |
| References | Fausto N. J Hepatol 32; 19-31, 2000.
Lamszus K et al. Int J Dev Neurosci 17; 517-530, 1999. Stella MC and Comoglio PM. Int J Biochem Cell Biol 31; 1357-1367, 1999. |