DA3509S Placenta Growth Factor 1 (PlGF-1) HIS tagged antigen
2 µg / US$ 225
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Human Placenta Growth Factor 1 (PlGF-1) HIS tagged
Synonyms
PlGF1,
Product Description
Human Placenta Growth Factor 1 (PlGF-1) HIS tagged
Properties
| Presentation | Purified |
| Species | Human |
| Purity | >95% |
| Source | Insect cells |
| Molecular Weight | 38 kD |
| Catalog Number | DA3509S |
| Quantity | 2 µg |
| Price | US$ 225 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  DA3509S.pdf |
Datasheet Extract
| Alternate names | Placenta Growth Factor 1 (PlGF-1) HIS tagged |
| Background | Human Placenta Growth Factor-1 (PlGF-1), a 19 kDa protein consisting of 131 amino acid residues and fused to a C-terminal His -tag (6x His), is produced as a homodimer. Human Placenta Growth Factor (PlGF) is a polypeptide growth factor and a member of the platelet-derived growth factor family but more related to vascular endothelial growth factor (VEGF). PlGF-1 acts only as a very weak mitogen for some endothelial cell types and as a potent chemoattractant for monocytes. The physiological function in vivo is still controversal. In several reports it was shown not to be a potent mitogen for endotehlial cells and not angiogenic in vivo by using different assays. Very recently it was shown by one investigator, that PlGF-1 from cell culture supernatants was angiogenic in the CAM assay and in the rabbit cornea assay. At least one high-affinity receptor for PlGF (FLT -1 or VEGF-R1) has been demonstrated in different primary cell types (e.g. human umbilical vein endothelial cells and monocytes) but PlGF does not bind to KDR/flk-1. Two different proteins can be generated by differential splicing of the human PlGF gene: PlGF-1 (131 aa native chain) and PlGF -2 (152 aa native chain). Both mitogens are secretable proteins, but PlGF -2 can bind to heparin with high affinity. PlGF-1 is a homodimer, but preparations of PlGF show some heterogeneity on SDS gels depending of the varying degrees of glycosylation. All dimeric forms posses a similar biological profile. There is good evidence that heterodimeric molecules between VEGF and PlGF exists and that they are biological active. Different cells and tissues (e.g. placenta) express PlGF-1 and PlGF-2 at different rates. A very related protein of PlGF is VEGF with about 53% homology and VEGF-B with similar biological activities. |
| Source | Insect cells |
| Format | State: Lyophilized purified fraction with BSA as stabilizer. Purity: > 95%, by SDS-PAGE and visualised by silver stain. Endotoxinlevel: < 0.1 ng per ug of PIGF-1 Reconstitution: Restore with 0.1 M acetic acid or PBS. |
| Description | Recombinant Human PIGF-1. Biological Activity: Measured by its ability to bind to immobilized rh-sFlt-1 in a functional ELISA. Recombinant human PlGF-1 can bind to immobilized rh-sFlt-1 (100 ng/well) with a linear range at 0.5-10 ng/ml. Molecular weight: 38 kDa |
| Add. Information | Always centrifuge vials before opening! |
| Storage | Store lyophilized human PIGF-1 at -20°C.
Reconstituted PIGF-1 should be stored in working aliquots at -20°C to -70°C. Avoid repeated freeze-thaw cycles! Shelf life: One year from despatch. |