DA3510 Placenta Growth Factor 2 (PlGF-2) antigen
see all 3 Placenta Growth Factor 2 products
5 µg / US$ 325
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Human Placenta Growth Factor 2 (PlGF-2)
Synonyms
PlGF2,
Product Description
Human Placenta Growth Factor 2 (PlGF-2)
Properties
| Presentation | Purified |
| Species | Human |
| Purity | >80% |
| Source | Insect cells |
| Molecular Weight | 44 kD |
| Catalog Number | DA3510 |
| Quantity | 5 µg |
| Price | US$ 325 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  DA3510.pdf |
Datasheet Extract
| Alternate names | Placenta Growth Factor 2 |
| Background | Human Placenta Growth Factor-2 (PlGF-2), a 22 kDa protein consisting of 152 amino acid residues is produced as a homodimer. PlGF is a polypeptide growth factor and a member of the platelet-derived growth factor family but more related to vascular endothelial growth factor (VEGF). PlGF acts only as a weak mitogen for those cell types possessing receptors for binding (e.g. vascular endothelial cells). At least one high-affinity receptor for PlGF (FLT -1 or VEGF-R1) has been demonstrated in different primary cell types (e.g. human umbilical vein endothelial cells and monocytes). In addition to its action as a weak mitogen it is also a chemoattractant for monocytes and endothelial cells. Two different proteins are generated by differential splicing of the human PlGF gene: PlGF-1 (131 aa native chain) and PlGF -2 (152 aa native chain). Both mitogens are secretable proteins, but PlGF-2 can bind to heparin with high affinity. PlGF is apparently a homodimer, but preparations of PlGF show some heterogeneity on SDS gels depending of the varying degrees of glycosylation.
All dimeric forms posses similar biologcal activities. If PlGF is angiogenic in vivo is not clear. However, heterodimers between VEGF and PlGF are mitogenic for endothelial cells and have strong angiogenic activity in vivo (e.g. in the CAM assay or in the cornea pocket assay). Different cells and tissues (e.g. placenta) express PlGF-1 and PlGF-2 at different rates. A very related protein of PlGF is VEGF with about 53% homology and VEGF-B with similar biological activities. |
| Source | Insect cells . |
| Format | State: Lyophilized with BSA as stabilizer. Purity: 80% by SDS-PAGE and visualised by silver stain. Endotoxinlevel: < 0.1 ng per µg of PIGF-2. Reconstitution: Restore with 0.1 M acetic acid or PBS. |
| Description | Recombinant Human PIGF-2. Biological Activity: Measured by its ability to bind to immobilized rh-sFlt-1 in a functional ELISA. Recombinant human PlGF-2 can bind to immobilized rh-sFlt-1 (100 ng/well) with a linear range at 0.3-10 ng/mL. Molecular weight: 44 kDa |
| Add. Information | Centrifuge vial before opening!
|
| Storage | Store lyophilized human PIGF-2 at -20°C.
Reconstituted PIGF-2 should be stored in working aliquots at -20°C to -70°C. Avoid repeated freeze-thaw cycles! Shelf life: One year from despatch. |