DA3511 Stem Cell Factor (SCF) antigen
see all 31 Stem Cell Factor products
2 µg / US$ 225
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Human Stem Cell Factor (SCF)
Product Description
Human Stem Cell Factor (SCF)
Properties
| Presentation | Purified |
| Species | Human |
| Purity | >95% |
| Source | Insect cells |
| Molecular Weight | 18.4 kD |
| Catalog Number | DA3511 |
| Quantity | 2 µg |
| Price | US$ 225 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  DA3511.pdf |
Datasheet Extract
| Background | Stem Cell Factor is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. This pleiotropic cytokine, alternately known as mast cell growth factor (MGF) and steel-factor (SLF), plays essential roles in gametogenesis, melanogenesis and early stages of hematopoiesis. In vitro and in vivo, SCF can stimulate the proliferation of mature, as well as the proliferation and maturation of immature, mast cells. On purified primitive human and mouse hematopoietic precursors, SCF acts in a synergistic manner with various growth factors, such as IL-1, IL-3, IL-6, IL-7, and Epo, to induce myeloid, erythroid and lymphoid lineage colony formation. The finding that SCF is also expressed in the nervous system suggests a possible role for SCF in the development of the nervous system. The cDNA sequences for human, mouse and rat SCF encode transmembrane proteins which are composed of a signal peptide, a 189 amino acid extracellular domain, a hydrophobic transmembrane domain and an intracellular domain. Native SCF can exist either as the membrane bound form or as a soluble form consisting of the first 164 or 165 amino acids of the extracellular domain. The soluble form is believed to be a proteolytic cleavage product of the transmembrane protein. Both the soluble and the transmembrane form of SCF have growth factor activities. Native soluble SCF is a heavily N- and O-glycosylated protein which exists as a non-covalently associated dimer in solution. All four cysteine residues of SCF monomers are involved in int ramolecular disulfide bonds.
Murine or rat soluble SCF is highly homologous to human soluble SCF (approximately 80%). Whereas both rat and mouse SCF are active on human cells, the human protein is much less active on mouse or rat cells. |
| Source | Insect cells |
| Format | State: Lyophilized Purity: 95% by SDS-PAGE and visualised by silver stain BufferSystem: PBS, pH 7.4, without stabilizer Endotoxinlevel: < 0.1 ng per µg of SCF Reconstitution: Centrifuge the vial prior to opening. Restore in 10 mM acetic acid to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for one week or at -20°C for future use. |
| Description | Stem Cell Factor (SCF) is a protein consisting of 164 amino acid residues (Asp26-Ala189) and fused to a C-terminal His-tag (6x His) Biological Activity: Human SCF is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is typically 2 - 5 ng/ml. Molecular weight: 18.4 kDa |
| Storage | The lyophilized human SCF, though stable at room temperature, is best stored desiccated below 0°C.
Reconstituted human SCF should be stored in working aliquots at -20°C. Avoid repeated frezing and thawing. Shelf life: one year from despatch. |