DA3512S Vascular endothelial growth factor A (Isoform 121) antigen
see all 114 Vascular endothelial growth factor A products
2 µg / US$ 235
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Human Vascular endothelial growth factor A (Isoform 121)
Synonyms
VEGFA, VEGF, VEGF-A, Vascular permeability factor, VPF,
Product Description
Human Vascular endothelial growth factor A (Isoform 121)
Properties
| Presentation | Purified |
| Species | Human |
| Purity | >90% |
| Source | Insect cells |
| Molecular Weight | 36 kD |
| Catalog Number | DA3512S |
| Swiss Prot. No. | P15692 |
| Quantity | 2 µg |
| Price | US$ 235 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  DA3512S.pdf |
Datasheet Extract
| Alternate names | VEGF 121 Isoform |
| Background | VEGF is a polypeptide growth factor and a member of the platelet-derived growth factor family. It is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. Two high-affinity tyrosine kinase receptors for VEGF121 have been identified, VEGFR-1 (FLT-1), and VEGFR-2 (KDR). Consistent with the endothelial cell-specific action of VEGF121, expression of both receptor genes has been found predominantly but not exclusively on endothelial cells. Expression of VEGFR-1 was also found on human monocytes, neutrophils (PMNs), bovine brain pericytes and villous and extravillous trophoblasts. In addition to its action as a mitogen it is a potent vascular permeability factor (VPF) in vivo, and a chemoattractant molecule for monocytes and endothelial cells. Five different proteins are generated by differential splicing: VEGF121, VEGF145, VEGF165, VEGF189 and VEGF206. The most abundant form is VEGF165. Whereas VEGF121 and VEGF165 are secreted proteins, VEGF145, VEGF189 and VEGF206 are strongly cell-associated. The isoforms VEGF145, VEGF165 and VEGF189 bind to heparin with high affinity. VEGF121 is apparently a homo-dimer, but preparations of VEGF show some heterogeneity on SDS gels depending on the secretion of different forms and the varying degrees of glycosylation. All dimeric forms posses similar biological activities but the bioavailability is very different. There is good evidence that heterodimeric molecules between the different isoforms exist and that different cells and tissues express different VEGF isoforms. The other members of this increasing growth factor family are VEGF-B, -C, -D, -E and PlGF. |
| Immunogen | Swiss Num.: P15692 |
| Source | Insect cells |
| Format | State: Lyophilized without buffer and stabilizer. Purity: > 90%, by SDS-PAGE and visualised by silver stain. Endotoxinlevel: < 0.1 ng per ug of VEGF. Reconstitution: Restore in PBS or medium containing at least 0.1% human or bovine serum albumin to a concentration not lower than 50 µg/ml. |
| Description | Human Vascular Endothelial Growth Factor121 (VEGF121)is a 18 kDa protein consisting of 121 amino acid residues, produced as a homodimer. Biological Activity: The ED50 for stimulation of 3H-thymidine incorporation and cell proliferation by human umbilical vein endothelial cells for VEGF121 has been determined to be in the range of 2-5 ng/ml. Specific Activity: 5 x 10e5 units/mg Molecular weight: ~ 36 kDa |
| Add. Information | Centrifuge vials before opening! |
| Storage | Store Lyophilized at -20°C to -70°C.
Reconstituted VEGF121 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles! Shelf life: One year from despatch. |
| References | 1. Breier et al., Dev 114:521, 1992.
2. Fiebig et al., Eur J 2. Biochem 211:19, 1993. 3. Flamme et al., Dev, Biol 162:699, 1995. 4. Kremer at al., Cancer Res 57:3852, 1997. |