DA3520 Vascular endothelial growth factor C antigen
see all 17 Vascular endothelial growth factor C products
5 µg / US$ 255
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rat Vascular endothelial growth factor C
Synonyms
Vegfc, VEGF-C, , VRP, Flt4 ligand, Flt4-L, Vascular endothelial growth factor-related protein
Product Description
Rat Vascular endothelial growth factor C
Properties
| Presentation | Purified |
| Species | Rat |
| Purity | >90% |
| Source | Insect cells |
| Molecular Weight | 24 kD |
| Catalog Number | DA3520 |
| Swiss Prot. No. | P49767 |
| Quantity | 5 µg |
| Price | US$ 255 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  DA3520.pdf |
Datasheet Extract
| Background | VEGF-C, also known as Vascular Endothelial Growth Factor Related Protein (VRP), is a recently discovered VEGF growth factor family member that is most closely related to VEGF-D. The rat VEGF-C cDNA encodes a pre-pro-protein of 416 amino acids residues. It is almost identical to the mouse VEGF-C protein. Similar to VEGF-D, VEGF-C has a VEGF homology domain spanning the middle third of the precursor molecule and long N- and C-terminal extensions. In adults, VEGF-C is highly expressed in heart, placenta, ovary and small intestine. Recombinant rat VEGF-C, lacking the N- and C-terminal extensions and containing only the middle VEGF homology domain, forms primarily non-covalently linked dimers. This protein is a ligand for both VEGFR-2/KDR and VEGFR-3/FLT-4. Since VEGFR-3 is strongly expressed in lymphatic endothelial cells, it has been postulated that VEGF-C is involved in the regulation of the growth and/or differentiation of lymphatic endothelium. Although recombinant rat VEGF-C is also a mitogen for vascular endothelial cells, it is much less potent than VEGF-A. The recombinant rat VEGF-C contains 129 amino acids residues and was fused to a His-tag (6x His) at the C-terminal end. As a result of glycosylation VEGF-C migrates as an 18-24 kDa protein in SDS-PAGE under reducing conditions. |
| Immunogen | Swiss Num.: P49767 |
| Source | Insect cells |
| Format | State: Lyophilized, containing BSA as a stabilizer Purity: > 90%, by SDS-PAGE and visualised by silver stain Endotoxinlevel: < 0.1 ng per µg of VEGF-C152S Reconstitution: The lyophilized VEGF-C152S is soluble in water and most aqueous buffers. Reconstitute in PBS or medium to a concentration not lower than 50 µg/ml. |
| Description | VEGF-C152S is a point mutant generated by the replacement of the second conserved Cys residue of the recombinant processed VEGF-C by a Ser residue. VEGF-C152S is analog to the human VEGF-C156S mutant and only active toward VEGFR-3/FLT-4 but, unlike wild type VEGF-C, is unable to bind to and to activate signalling through VEGFR-2/KDR. VEGF-C152S was inactive in the vascular permeability assay and did not increase migration of the capillary endothelial cells, indicating that these VEGF-like effects of VEGF-C require VEGFR-2 binding. Biological Activity: Measured by its ability to stimulate phosphorylation of the VEGFR-3/FLT-4 receptor in porcine aortic endothelial cells (PAE/FLT-4 cells). The ED50 for this effect is typically 150-300 ng/ml. Molecular weight: 18-24 kDa |
| Storage | Store lyophilized at -20°C to -70°C.
Reconstituted VEGF-C152S should be stored in working aliquots at -20°C. Avoid repeated freezing and thawing. Shelf life: six months from despatch. |
| References | Joukov et al., J Biol Chem 273 :6599, 1998.
Joukov et al., EMBO J 15:290, 1996. Olofsson et al., Curr Opin Biotech 10:528, 1999. Kirkin et al., Eur J Biochem 268:5530, 2001. |