DA3534 Vascular endothelial growth factor receptor 1 (Fc-Chimera) antigen
see all 46 Vascular endothelial growth factor receptor 1 products
10 µg / US$ 225
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Human Vascular endothelial growth factor receptor 1 (Fc-Chimera)
Synonyms
FLT1, FLT, FRT, FLT-1, VEGFR-1, VEGFR1, EC 2.7.10.1, Vascular permeability factor receptor, Tyrosine-protein kinase receptor FLT, Flt-1, Tyrosine-protein kinase FRT, Fms-like tyrosine kinase 1,
Product Description
Human Vascular endothelial growth factor receptor 1 (Fc-Chimera)
Properties
| Presentation | Purified |
| Species | Human |
| Purity | >95% |
| Source | Insect cells |
| Molecular Weight | 130 kD |
| Catalog Number | DA3534 |
| Swiss Prot. No. | P17948 |
| Quantity | 10 µg |
| Price | US$ 225 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  DA3534.pdf |
Datasheet Extract
| Alternate names | FLT-1 |
| Background | Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs).
They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), and VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes. All VEGF-receptors have seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular split tyrosine kinase domain. VEGFR-2 has a lower affinity for VEGF than the Flt-1 receptor, but a higher signalling activity. Mitogenic activity in endothelial cells is mainly mediated by VEGFR-2 leading to their proliferation. Differential splicing of the flt-1 gene leads to the formation of a secreted, soluble variant of VEGFR-1 (sVEGFR-1). No naturally occurring, secreted forms of VEGFR-2 have so far been reported. The binding of VEGF165 to VEGFR-2 is dependent on heparin. |
| Immunogen | Swiss Num.: P17948 |
| Source | Insect cells |
| Format | State: Lyophilized Purity: > 95%, by SDS-PAGE and visualised by silver stain BufferSystem: PBS, pH 7.4, without stabilizer Endotoxinlevel: < 0.1 ng per ug of sVEGFR-1 Reconstitution: The lyophilised sVEGFR-1/Fc is soluble in water and most aqueous buffers. The product should be reconstituted in PBS or medium to a concentration not lower than 50 ?g/ml. |
| Description | Recombinant human soluble Vascular Endothelial Growth Factor Receptor-1 (sVEGFR-1D1-7) was fused with the Fc part of human IgG1.
The recombinant mature sVEGFR-1D1-7/Fc is a disulfide-linked homodimeric protein. The sVEGFR-1D1-7/Fc monomers have a mass of approximately 130 kDa. The soluble receptor protein consists of all 7 extracellular domains (Met1-Thr751), which contain all the information necessary for high affinity ligand binding. Biological Activity: The activity of sVEGFR-1/Fc was determined by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells. The ED50 for this effect is typically 10-30 ng/ml. |
| Storage | Lyophilized samples are stable for one year from despatch at -20°C to -70°C.
Reconstituted sVEGFR-1/Fc should be stored in working aliquots at -20°C. Avoid repeated freezing and thawing. |
