DA3541 Vascular endothelial growth factor receptor 1 (D1-D3) antigen
see all 46 Vascular endothelial growth factor receptor 1 products
5 µg / US$ 275
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Human Vascular endothelial growth factor receptor 1 (D1-D3)
Synonyms
FLT1, FLT, FRT, FLT-1, VEGFR-1, VEGFR1, EC 2.7.10.1, Vascular permeability factor receptor, Tyrosine-protein kinase receptor FLT, Flt-1, Tyrosine-protein kinase FRT, Fms-like tyrosine kinase 1,
Product Description
Human Vascular endothelial growth factor receptor 1 (D1-D3)
Properties
| Presentation | Purified |
| Species | Human |
| Purity | >90% |
| Source | Insect cells |
| Molecular Weight | 45 kD |
| Catalog Number | DA3541 |
| Swiss Prot. No. | P17948 |
| Quantity | 5 µg |
| Price | US$ 275 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  DA3541.pdf |
Datasheet Extract
| Alternate names | sVEGFR-1(D1-D3), sFlt-1 (D3) |
| Background | Recombinant human soluble Vascular Endothelial Growth Factor Receptor-1 domain D1-3 (sVEGFR-1 D1-3) is produced as a non-chimeric protein in a monomeric form. The soluble receptor protein contains only the first 3 extracellular domains, which contain all the information necessary for binding of VEGF. The receptor monomers have a mass of approximately 45 kDa containing 352 amino acid residues.
Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVE supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA. The biological functions of sVEGFR-1 still are not clear, but it seems to be an endogenous regulator of angiogenesis, binding VEGF with the same affinity as the full-length receptor. |
| Immunogen | Swiss Num.: P17948 |
| Source | Insect cells |
| Format | State: Lyophilized without buffer and astabilizer. Purity: 95%, by SDS-PAGE and visualised by silver stain. Endotoxinlevel: < 0.1 ng per µg of VEGF. Reconstitution: Restore in water to a concentration of 100 ng/ml. |
| Description | Recombinant Human sFlt-1 (D3). Biological Activity: The activity of sVEGFR-1 (D1-3) was determined by its ability to abolish the binding of iodinated VEGF to solid surfaces or cell surfaces receptors, and in Far-Western and cross-linking experiments with iodinated VEGF. Specific Activity: 2.5 x 10e5 units/mg Molecular weight: 45 kDa |
| Add. Information | Centrifuge vials before opening! |
| Storage | Store the Lyophilized samples at -20°C to -70°C.
Reconstituted sVEGFR-1(D1-3) should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles! Shelf life: One year from despatch |
| References | 1. Barleon et al., 1997, J Biol Chem 272:10382-8.
2. Röckl et al., 1998, Exp Cell Res, 241:161-170. |
