DM1204 CD66A, CD66C, CD66D, CD66E and CEACAM4 antibody
see related secondary antibodies
0.1 mg / US$ 435
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti CD66A, CD66C, CD66D, CD66E and CEACAM4 D14HD11
Synonyms
CEACAM1, BGP, BGP1, CD66a, BGP-1, Biliary glycoprotein 1, , Carcinoembryonic antigen-related cell adhesion molecule 1
Product Description
Mouse anti CD66A, CD66C, CD66D, CD66E and CEACAM4 D14HD11, Presentation: Purified. Product is tested for Frozen sections ( C ), Enzyme Immunoassay ( E ), Flow cytometry ( F ), Immunofluorescence ( IF ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Enzyme Immunoassay ( E ), Flow cytometry ( F ), Immunofluorescence ( IF ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | D14HD11 |
| Catalog Number | DM1204 |
| Swiss Prot. No. | P13688 |
| Quantity | 0.1 mg |
| Price | US$ 435 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  DM1204.pdf |
Datasheet Extract
| Concentration | 2.0 mg/ml |
| Background | CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family (13). It consists of seven CEACAM (CEACAM1, CEACAM3-CEACAM8) and 11 pregnancy-specific glyco-protein (PSG1-PSG11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains (Fig. 2). CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor (Fig. 2). CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/ CEACAM5 in tumors of epithelial origin is the basis of ist wide-spread use as a tumor marker (5). The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria (13).
|
| Immunogen | CEA partially purified from a perchloric acid extract from liver metastases of colonic tumors (6).
Swiss Num.: P13688 |
| Format | State: Liquid IgG fraction Purification: Protein G affinity chromatography BufferSystem: PBS, pH 7.2 |
| Applications | D14HD11 has been tested for the use in flow cytometry (The antibody is routinely tested by flow cytometry on BOSC23 cells transiently transfected with a CEACAM5 expression vector), cell-ELISA, ELISA, Western blots, immunoprecipitation, immunofluorescence (Fig. 3, kindly provided by Dr. J. Schneider, Universität Freiburg) and immunohistology (cryosections). For various aplications see list of references. |
| Specificity | D14HD11 is a broadly reactive antibody which reacts with high affinity (affinity to solid phase bound CEACAM5: 2 x 10e10 L/Mol [6]) with most of the CEACAM molecules (except CEACAM7 and CEACAM8) transiently expressed on the cell surface of transfected BOSC23 cells.
D14HD11 was included and characterized in the studies from the VIth Leucocyte Typing Workshop. It reacts with the CEACAM family N-domain. |
| Storage | Store the antibody at 2-8°C.
Shelf life: one year from despatch. |
| References | 1. Billker O, Popp A, Brinkmann V, Wenig G, Schneider J, Caron E, Meyer TF (2002). Distinct mechanisms of internalization of Neisseria gonorrhoeae by members of the CEACAM receptor family involving Rac1- and Cdc42-dependent and -independent pathways. EMBO J. 21: 560-571.
2. Boulton IC and Gray-Owen SD (2002). Neisserial binding to CEACAM1 arrests the activation and proliferation of CD4+ T lymphocytes. Nat Immunol. 3: 229-236. 3. Grunert F, Stocks SC, Nagel G., Zimmermann W, Thompson JA, Jantscheff P and Kromer B (1996). CD66 family Workshop: Binding of myeloid blind panel antibodies and CD66 Subsection antibodies to HeLa transfectants expressing individual CD66 molecules. In Leukocyte Typing VI: White cell Differentiation Antigens (T. Kishimoto et al., eds.), Garland Publishing Inc., New York and London, pp. 1012-1025. 4. Guignot J, Peiffer I, Bernet-Camard MF, Lublin DM, Carnoy C, Moseley SL and Servin AL (2000). Recruitment of CD55 and CD66e brush border-associated glycosylphosphatidylinositol-anchored proteins by members of the Afa/Dr diffusely adhering family of Escherichia coli that infect the human polarized intestinal Caco-2/TC7 cells. Infect Immun. 68: 3554-3563. 5. Hammarström S (1999). The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues. Semin. Cancer Biol. 9: 67-81. 6. Jantscheff P, Bottger V, Price M, Micheel B, Kaiser G, Zotter S, Kotzsch M, Grossmann H, Karsten UF (1991). Production and characterization of monoclonal antibodies against carcinoembryonic antigen (CEA). Biomed. Biochim. Acta. 50: 1261-1274. 7. Krop-Watorek A, Laskowska A, Salwa J, Klopocki AG, Grunert F, Ugorski M. (1999). CEA-related proteins on human urothelial cell lines of different transformation grades. Cancer Lett. 139: 15-22. 8. McCaw SE, Schneider J, Liao EH, Zimmermann W and Gray-Owen SD (2003). Immunoreceptor tyrosine-based activation motif phosphorylation during engulfment of Neisseria gonorrhoeae by the neutrophil-restricted CEACAM3 (CD66d) receptor. Mol Microbiol. 49: 623-637. 9. Muenzner P, Dehio C, Fujiwara T, Achtman M, Meyer TF, Gray-Owen SD (2000). Carcinoembryonic antigen family receptor specificity of Neisseria meningitidis Opa variants influences adherence to and invasion of proinflammatory cytokine-activated endothelial cells. Infect Immun. 68: 3601-3607. 10. Muenzner P, Naumann M, Meyer TF, Gray-Owen SD (2001). Pathogenic Neisseria trigger expression of their carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1; previously CD66a) receptor on primary endothelial cells by activating the immediate early response transcription factor, nuclear factor-kappaB J Biol Chem. 276: 24331-24340. 11. Popp A, Dehio C, Grunert F, Meyer TF and Gray-Owen SD (1999). Molecular analysis of neisserial Opa protein interactions with the CEA family of receptors: identification of determinants contributing to the differential specificities of binding. Cellular Micorbiology 1, 169-181. 12. Watt SM, Teixeira AM, Zhou GQ, Doyonnas R, Zhang Y, Grunert F, Blumberg RS, Kuroki M, Skubitz KM, Bates PA (2001). Homophilic adhesion of human CEACAM1 involves N-terminal domain interactions: structural analysis of the binding site. Blood. 98: 1469-1479. 13. Zimmermann W (2002). Carcinoembryonic antigen. In Wiley Encyclopedia of Molecular Medicine (T. Creighton, ed.), John Wiley & Sons Inc., New York, USA, pp. 459-462. |
| Pictures | Fig. 1: Specificity testing of
D14HD11. BOSC cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3, CEACAM5-CEACAM8 or a recombinant transmembrane-anchored PSG1 fusion protein. Recognition of CEACAM4 was tested on CHO cells stably transfected with a CEACAM4 expression vector. Expression of the constructs was confirmed with monoclonal antibodies known to recognise the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: CAC2; CEACAM8: 80H3; PSG1: BAP1; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified D14HD11 was tested on all CEACAM transfectants. A positive signal was obtained with CEACAM1, CEACAM3, CEACAM4, CEACAM5 and CEACAM6 expressing cells (red curves) |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 315 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 305 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 325 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 465 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 465 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'DM1204 CD66A, CD66C, CD66D, CD66E and CEACAM4'.