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Formalin fixed paraffin embedded human lung squamous cell carcinoma stained with EGFR antibody. DM267

 

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DM267  EGFR antibody

see related secondary antibodies
see all 203 EGFR products
1 ml / US$ 575
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Mouse anti EGFR 111.6

Synonyms

Epidermal Growth Factor Receptor, ERBB1, Receptor tyrosine-protein kinase ErbB-1

Product Description

Mouse anti EGFR 111.6, Presentation: Purified. Product is tested for Frozen sections ( C ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )

Properties

ApplicationsFrozen sections ( C ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
ReactivityHuman ( Hu )
PresentationPurified
HostMouse
IsotypeIgG1
Clone111.6
Catalog NumberDM267
Swiss Prot. No.P00533
Quantity1 ml
PriceUS$ 575
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
DM267.pdf

Datasheet Extract

BackgroundIts epitope maps in the extracellular domain of EGFR. Overexpression of EGFR has been reported in tumors of breast, brain, bladder, lung, gastric, head and neck, esophagus, cervix, vulva, ovary and endometrium. EGFR is over expressed in squamous cell carcinoma.
ImmunogenBALB/C mice were injected with extracellular domain of human recombinant EGFR protein.
Swiss Num.: P00533
Format
State: Liquid Ig fraction
BufferSystem: containing sodium azide as preservative.
ApplicationsImmunoprecipitation: 1:50.
Western Blot: 1:50 to 1:100.
Immunohistochemistry on paraffin sections:
diluted of 1:25-1:50 in an ABC method (30 minutes at room temperature or overnight at 4°C)
Formalin fixed paraffin embedded tissue sections require digestion with Protease XXV at 1mg/ml PBS for 5 minutes at 37°C.
Recommended Positive Control: Placenta
SpecificityThis antibody is specific to 170 kD known as first member (EGF-Receptor) of type I Family of growth factor receptors. Cellular Localization: Cell membrane
Species: Human.
StorageStore the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
PicturesFormalin fixed paraffin embedded human lung squamous cell carcinoma stained with EGFR antibody. DM267

14 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1253B Mouse IgG (H&L)  
  Rabbit Biotin   2 mg / US$ 315
   
R1253F Mouse IgG (H&L)  
  Rabbit FITC   2 mg / US$ 295
   
R1253T Mouse IgG (H&L)  
  Rabbit TRITC   2 mg / US$ 295
   
R1253TR Mouse IgG (H&L)  
  Rabbit Texas Red   2 mg / US$ 305
   
R1253HRP Mouse IgG (H&L)  
  Rabbit HRP   2 mg / US$ 325
   
R1253AP Mouse IgG (H&L)  
  Rabbit AP   1 mg / US$ 335
   
R1457C2 Mouse IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / US$ 455
   
R1457C3 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / US$ 455
   
R1457C35 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / US$ 455
   
R1457C5 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / US$ 455
   
R1457C55 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / US$ 455
   
R1405R Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh  
  Goat PE   1 ml / US$ 465
   
R1403R Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins  
  Rabbit PE   1 ml / US$ 465
   
R1455R Mouse IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 465
   

Click here to see all secondary antibodies for 'DM267 EGFR'.