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Immunostaining of human lymphatic endothelial cells using anti-Podoplanin antibody

 

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DM3500  Podoplanin antibody

see related secondary antibodies
see all 14 Podoplanin products
0.2 ml / US$ 455
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Mouse anti Podoplanin 18H5

Synonyms

Angiogenesis,

Product Description

Mouse anti Podoplanin 18H5, Presentation: Supernatant. Product is tested for Frozen sections ( C ), Flow cytometry ( F ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )

Properties

ApplicationsFrozen sections ( C ), Flow cytometry ( F ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
ReactivityHuman ( Hu )
PresentationSupernatant
HostMouse
IsotypeIgG1
Clone18H5
Catalog NumberDM3500
Quantity0.2 ml
PriceUS$ 455
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
DM3500.pdf

Datasheet Extract

BackgroundPodoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. Podoplanin is a Mr 38,000 membrane mucoprotein that was originally detected on the surface of rat glomerular epithelial cells (podocytes) and was found to be linked to flattening of foot processes that occurs in glomerular diseases. Podoplanin shows features of a membrane mucoprotein with several conserved O-glycosylation sites. Currently, it is of unknown biological function. Because heavily O-glycosylated mucoproteins were identified recently as counterreceptors for selectins that mediate adhesion of inflammatory cells, it is possible that podoplanin plays a similar role in lymphatic endothelia.
Format
State: Liquid concentrated tissue culture supernatant containing 0.09% sodium azide as preservative
ApplicationsFlow cytometry (1:50).
Immunoprecipitation (1:50).
Immunohistochemistry on frozen and paraffin embedded sections (1:50 - 1:200).
Western blotting (>1:100.)
SpecificityThis antibody will detect Podoplanin on the surface of lymphatic endothelial cells and kidney podocytes.
Species: Human. This antibody is not reactive with mouse podoplanin.
StorageStore the antibody at 2-8°C for up to one month or at -20°C for longer (add 50% Glycerol). Avoid repeated freezing and thawing. Shelf life: one year from despatch.
PicturesImmunostaining of human lymphatic endothelial cells using anti-Podoplanin antibody

14 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1253B Mouse IgG (H&L)  
  Rabbit Biotin   2 mg / US$ 305
   
R1253F Mouse IgG (H&L)  
  Rabbit FITC   2 mg / US$ 285
   
R1253T Mouse IgG (H&L)  
  Rabbit TRITC   2 mg / US$ 285
   
R1253TR Mouse IgG (H&L)  
  Rabbit Texas Red   2 mg / US$ 295
   
R1253HRP Mouse IgG (H&L)  
  Rabbit HRP   2 mg / US$ 315
   
R1253AP Mouse IgG (H&L)  
  Rabbit AP   1 mg / US$ 325
   
R1457C2 Mouse IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / US$ 445
   
R1457C3 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / US$ 445
   
R1457C35 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / US$ 445
   
R1457C5 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / US$ 445
   
R1457C55 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / US$ 445
   
R1405R Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh  
  Goat PE   1 ml / US$ 455
   
R1403R Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins  
  Rabbit PE   1 ml / US$ 455
   
R1455R Mouse IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 455
   

Click here to see all secondary antibodies for 'DM3500 Podoplanin'.