DM3500P Podoplanin antibody
see related secondary antibodiessee all 14 Podoplanin products
0.1 mg / US$ 455
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Podoplanin 18H5
Synonyms
Angiogenesis,
Product Description
Mouse anti Podoplanin 18H5, Presentation: Purified. Product is tested for Frozen sections ( C ), Flow cytometry ( F ), Immunofluorescence ( IF ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Flow cytometry ( F ), Immunofluorescence ( IF ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | 18H5 |
| Catalog Number | DM3500P |
| Quantity | 0.1 mg |
| Price | US$ 455 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  DM3500P.pdf |
Datasheet Extract
| Immunogen | gp36 (podoplanin) expressed by MDCK cells |
| Format | State: Lyophilized IgG fraction Purification: Protein G affinity chromatography BufferSystem: PBS, pH 7.4, without preservatives Reconstitution: Restore in sterile water to a concentration of > 0.5 mg/ml. |
| Applications | FACS analysis (3-20 µg/ml).
Immuofluorescence (1-2 µg/ml). Western blot analysis (1-2 µg/ml). Immunohistochemistry on frozen and paraffin sections (6-30 µg/ml). |
| Specificity | This antibody reacts with Podoplanin (gp36). Species: Human. |
| Storage | The lyophilized IgG is stable at 2-8°C for one month and for one year when kept at -20°C.
When reconstituted in sterile water to a concentration of > 0.5 mg/ml the antibody is stable for six weeks at 2-8°C in the presence of a preservative. Reconstituted antibody can also be aliquotted and stored at -20°C to -70°C for 6 months without detectable loss of activity. Avoid repeated freezing and thawing. |
| References | Breiteneder-Geleff et al., Am. J. Pathol. 151:1141,1997
Zimmer et al., Biochem. J. 341:277, 1999 Schacht et al., EMBO J. 22:3546-56, 2003 |
| Protocols | Sample protocol for formalin-fixed paraffin-embedded sections (as a guide only)
1) Put Paraffin sections (2-4 µm) onto a Poly-Lysin object holder 2) Deparaffination: - Xylol (10 min) - Acetone (10 min) - Acetone / buffer mixture (10 min) - Rinse 3x with wash buffer 3) Microwave: boil up 3x 10 min in the appropriate buffer (put the whole glass curette into the microwave) a) EDTA buffer: 0,1 M EDTA in aqua dest, pH 8,0 b) Citrate buffer/10 mM, pH 6,0 A: citric acid, 19,2 g in 1L aqua dest B: Na-Citrate, 29,41 g in 1L aqua dest 9 ml stock solution A + 50 ml stock solution B, in 500 ml aqua dest (pH adjustment only in solution B) Cool down the sections to RT for 25 min in wash buffer 4) Addition of primary ABs (dilute e.g. in ready to use buffer from DAKO [S3022]) - Shortly drip off the sections on paper (take 3-4 sections at once) - Dust off the liquid beside the sections - Add primary Abs - incubate 0.5 h at RT - Rinse 3x with wash buffer - Shortly drip off the sections 5) Addition of the by-pass AB (monoclonal mouse-anti-rabbit IgG, e.g. DAKO [M0737] 1: 250) - incubate 0.5 h at RT - Rinse 3x with wash buffer - Shortly drip off the sections 6) Addition of anti-mouse-IgG from rabbit 1: - Incubate 0.5 h at RT - Rinse 3x with wash buffer - Shortly drip off the sections 7)vAddition of the APAAP complexes - Incubate 0.5 h at RT - Rinse 3x with wash buffer - Shortly drip off the sections 8)Addition of the Chromogens (NaphtolAS-MXPhosphat plus FastredTR-Salz) - Incubate for 25 min at RT - Rinse 3x with wash buffer - Shortly drip off the sections - Counterstain: Hämalaun - 45 sec - rinse 3x 5 min in lukewarm tap water - rinse 1x 5 min in dest. H2O 9) Cover the sections in Glycergel (DAKO) Note: For steps 6,7,8 use kit from DAKO: Universal-DAKO APAAP Kit Mouse Note: After each step the sections could stay in the buffer! Wash buffer (for 40 L): 351g NaCl, 274g Tris-HCL, 36g Tris-Base |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'DM3500P Podoplanin'.
