DM3501 Podoplanin antibody
see related secondary antibodiessee all 14 Podoplanin products
200 µl / US$ 455
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Hamster anti Podoplanin 811
Synonyms
Angiogenesis,
Product Description
Hamster anti Podoplanin 811, Presentation: Purified. Product is tested for Frozen sections ( C ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB ) |
| Reactivity | Mouse ( Ms ) |
| Presentation | Purified |
| Host | Hamster |
| Isotype | IgG |
| Clone | 811 |
| Catalog Number | DM3501 |
| Quantity | 200 µl |
| Price | US$ 455 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  DM3501.pdf |
Datasheet Extract
| Concentration | 0.5 mg/ml |
| Background | Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. Podoplanin is a Mr 38,000 membrane mucoprotein that was originally detected on the surface of rat glomerular epithelial cells (podocytes) and was found to be linked to flattening of foot processes that occurs in glomerular diseases. Podoplanin shows features of a membrane mucoprotein with several conserved O-glycosylation sites. Currently, it is of unknown biological function. Because heavily O-glycosylated mucoproteins were identified recently as counterreceptors for selectins that mediate adhesion of inflammatory cells, it is possible that podoplanin plays a similar role in lymphatic endothelia.
|
| Format | State: IgG fraction Purification: Protein G affinity chromatography BufferSystem: PBS |
| Applications | Immunoprecipitation (1:50).
Immunohistochemistry on frozen and paraffin embedded sections (1:50 - 1:200). Western blotting (>/= 1:100; the antibody will detect a 36-40 kD band). |
| Specificity | This antibody will detect the extracellular domain of Podoplanin on the surface of lymphatic endothelial cells and some epithelial cell types. Species: Mouse. The antibody is not reactive with human podoplanin. |
| Storage | Store the antibody at 2-8°C for up to one month or at -20°C for longer (add 50% Glycerol).
Shelf life: one year from despatch. |
| Protocols | IMMUNOSTAINING ON FORMALIN FIXED PARAFFIN-EMBEDDED SECTIONS
1. Deparaffinize through xylenes, ethanol , and hydrate to water. 2. 1% hydrogen peroxide in methanol , 30-40 min., RT. 3. Wash 2x, dd water, wash in Tris buffer, pH 7.2, 5 min. each. 4. Proteinase K in 0.2M Tris, buffer, pH 7.2, 37°C, 36 µg/ml, 30 min. 5. Wash 3x, 5 min each: Tris buffer, pH 7.2, dd sterile water; TNT wash buffer. 6. Block in TNB blocking buffer, 30 min. RT. 7. Primary antibody: Hamster anti-mouse Podoplanin antibody 5-10 µg/ml in TNB buffer, overnight, 4°C. 8. Rinse in TNT wash buffer, 3x, 5 min. 9. Incubate with rabbit anti-syrian hamster IgG HRP conjugated, 1:100 in TNB buffer, 30 min. RT. Wash 3x. 10. Biotinylated-tyramide in amplification diluent, 1:50 , 7 minutes, RT Wash 3x. 11. Incubate in SA-HRP , 1:100 in TNB, 30 min, RT. Wash 3x. 12. DAB 13. Counter stain |
11 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1327B | Hamster IgG (H&L) | ||||||
| Rabbit | Biotin | 1.5 mg / US$ 315 | |||||
| R1327F | Hamster IgG (H&L) | ||||||
| Rabbit | FITC | 1.5 mg / US$ 295 | |||||
| R1327T | Hamster IgG (H&L) | ||||||
| Rabbit | TRITC | 1.5 mg / US$ 295 | |||||
| R1327TR | Hamster IgG (H&L) | ||||||
| Rabbit | Texas Red | 1.5 mg / US$ 315 | |||||
| R1327HRP | Hamster IgG (H&L) | ||||||
| Rabbit | HRP | 1.5 mg / US$ 325 | |||||
| R1327AP | Hamster IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 345 | |||||
| R1437C2 | Hamster IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1437C3 | Hamster IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1437C35 | Hamster IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1437C5 | Hamster IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1437C55 | Hamster IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
Click here to see all secondary antibodies for 'DM3501 Podoplanin'.
