DP138-05 DFF45 / ICAD / DFFA antibody
see related secondary antibodiessee all 41 DFF45 / ICAD / DFFA products
0,5 ml / US$ 375
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti DFF45 / ICAD / DFFA
Synonyms
Inhibitor of CAD, DFF-45, DFF1, , DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit
Product review
Please read our product review about DFF45 / ICAD / DFFA: Antibodies to DNA Fragmentation Factor: DFF40 (CAD) and DFF45 (ICAD).
Product Description
Rabbit anti DFF45 / ICAD / DFFA , Presentation: Purified. Product is tested for Paraffin Sections ( P )
Properties
| Applications | Paraffin Sections ( P ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Rabbit |
| Catalog Number | DP138-05 |
| Swiss Prot. No. | O00273 |
| Quantity | 0,5 ml |
| Price | US$ 375 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  DP138-05.pdf |
Datasheet Extract
| Background | Apoptosis is induced through a number of cell death receptors and their ligands like TNF and TNF receptor. The signaling cascade is carried further by adaptor proteins containing death domains and by caspase family of proteins. DFF45 and DFF40 (ICAD and CAD) are two subunits of heterodimeric caspase activated DNAse. DFF45/ ICAD (Inhibitor of Caspase Activated DNAse) acts as a chaperon for appropriate folding of DFF40 and it also acts as inhibitor of DFF40. Caspase 3 upon activation cleaves DFF to release DFF40/CAD, which oligomerizes into a large active complex and eventually causes the degradation of DNA in nuclei. Hence cleavage of DFF45 is an important step in apoptosis. |
| Immunogen | A synthetic peptide derived from N-terminus of human DFF-45 protein. Swiss Num.: O00273 |
| Format | State: Liquid Ig fraction BufferSystem: 10 mM citrate buffer, pH 6.0 - containing sodium azide as preservative. |
| Applications | Immunohistochemistry on formalin-fixed paraffin embedded sections:
diluted of 1:50-1:100 in an ABC method (30 minutes at room temperature) Recommended Positive Control: Colon carcinoma |
| Specificity | This antibody reacts with human DFF-45.
Cellular Localization: Cytoplasmic Species: Human. |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Pictures | Formalin fixed paraffin embedded human Colon carcinoma stained with DFF-45 antibody. DP138 |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 305 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 295 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 295 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 305 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 305 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 335 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'DP138-05 DFF45 / ICAD / DFFA'.