DP2009 Fatty acid-binding protein 4 / FABP4 (adipocyte) antibody
see related secondary antibodiessee all 17 Fatty acid-binding protein 4 / FABP4 products
0.05 mg / US$ 485
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Fatty acid-binding protein 4 / FABP4
Synonyms
A-FABP, AFABP, Fatty acid-binding protein 4, ALBP,
Product Description
Rabbit anti Fatty acid-binding protein 4 / FABP4 , Presentation: Purified. Product is tested for Frozen sections ( C ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Rabbit |
| Catalog Number | DP2009 |
| Swiss Prot. No. | P15090 |
| Quantity | 0.05 mg |
| Price | US$ 485 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  DP2009.pdf |
Datasheet Extract
| Alternate names | AFABP, FABP4 |
| Concentration | 1.0 mg/ml |
| Background | Adipocyte fatty acid binding protein AFABP is a 15 kDa member of the intracellular fatty acid binding protein (FABP) family, which is known for the ability to bind fatty acids and related compounds (bile acids or retinoids) in an internal cavity. AFABP is expressed in a differentiation-dependent fashion in adipocytes and is a critical gene in the regulation of the biological function of these cells . In mice, targeted mutations in AFABP provide significant protection from hyperinsulinemia and insulin resistance in the context of both dietary and genetic obesity. Adipocytes obtained from AFABP-deficient mice also have reduced fficiency of lipolysis in vitro and in vivo, and these mice exhibited moderately improved systemic dyslipidemia. Recent studies also demonstrated AFABP expression in macrophages upon differentiation and activation. In these cells, AFABP modulates inflammatory responses and cholesterol ester accumulation, and total or macrophage-specific AFABP deficiency confers dramatic protection against atherosclerosis in the apoE-/- mice. These results indicate a central role for AFABP in the development of major components of the metabolic syndrome through its distinct actions in adipocytes and macrophages. |
| Immunogen | The immunization antigen (14.7 kDa) is a protein containing 131 AA of recombinant Human FABP4 and one extra AA, N-terminal methionin (highlighted). Swiss Num.: P15090 AA Sequence: MCDAFVGTWK LVSSENFDDY MKEVGVGFAT RKVAGMAKPN MIISVNGDVI TIKSESTFKN TEISFILGQE FDEVTADDRK VKSTITLDGG VLVHVQKWDG KSTTIKRKRE DDKLVVECVM KGVTSTRVYE RA |
| Format | State: Lyophilized purified IgG Purification: Immunoaffinity chromatography BufferSystem: 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE Reconstitution: Restore with 0.05 ml of deionized water. |
| Applications | Immunohistochemistry on frozen sections.
Western Blot. |
| Specificity | This antibody reacts to Adipocyte fatty acid binding protein. Species: Human. |
| Storage | Store lyophilized (preferably in a desiccator) at -20°C and in aliquots at -20°C to -80°C.
Reconstituted antibody can be stored at 2-8°C for a limited period of time; it does not show decline in activity after two weeks at 2-8°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Xu A, Wang Y, Xu JY, Stejskal D, Tam S, Zhang J, Wat NM, Wong WK, Lam KS . Adipocyte fatty acid-binding protein is a plasma biomarker closely associated with obesity and metabolic syndrome. Clin Chem . Mar;52(3):405-13 (2006)
See: http://www.clinchem.org/cgi/content/abstract/52/3/405 2. Prinsen CF, de Bruijn DR, Merkx GF, Veerkamp JH. Assignment of the human adipocyte fatty acid-binding protein gene (FABP4) to chromosome 8q21 using somatic cell hybrid and fluorescence in situ hybridization techniques. 3. van Dongen MJ, Uppenberg J, Svensson S, Lundback T, Akerud T, Wikstrom M, Schultz J. Structure-based screening as applied to human FABP4: a highly efficient alternative to HTS for hit generation. 4. Witthuhn BA, Bernlohr DA. Upregulation of bone morphogenetic protein GDF-3/Vgr-2 expression in adipose tissue of FABP4/aP2 null mice. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 305 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 295 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 295 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 305 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 305 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 335 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 455 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 455 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 455 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 455 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 455 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'DP2009 Fatty acid-binding protein 4 / FABP4 '.