GTX23871 MCF-7 whole cell lysate
100 µg / no delivery possible
Quick Overview
MCF-7 whole cell lysate
Product Description
MCF-7 whole cell lysate
Properties
| Catalog Number | GTX23871 |
| Quantity | 100 µg |
| Price | no delivery possible |
| Delivery | Europe only |
| Manufacturer | GeneTex Inc. |
| Datasheet |  http://www.genetex.com/co ... |
Datasheet Extract
| MCF-7 whole cell lysate | |
| Concentration | 2 mg/ml |
| Background | MCF7 whole cell lysate was prepared by homogenization in modified RIPA buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 1 mM EDTA, 1 mM PMSF, 5 ug/ml Aprotinin, 1 ug/ml Pepstatin-A, 2 ug/ml Leupeptin, 1 mM Na3VO4, 1 mM NaF). Debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol. |
| Format | State: Supplied as whole cell lysate, in 1 x SDS sample buffer containing 5% beta-mercaptoethanol. Purification: n/a Purity: n/a BufferSystem: Whole lysate in 1 X SDS containing 5% beta-mercaptoethanol |
| Applications | WB |
| Storage | Keep as concentrated solution. Aliquot and store at -20 |