GTX27275 Mouse spleen lysate (7 days old)
see all 3 Mouse spleen lysate products
100 µg / no delivery possible
Quick Overview
Mouse spleen lysate (7 days old)
Product Description
Mouse spleen lysate (7 days old)
Properties
| Catalog Number | GTX27275 |
| Quantity | 100 µg |
| Price | no delivery possible |
| Delivery | Europe only |
| Manufacturer | GeneTex Inc. |
| Datasheet |  http://www.genetex.com/co ... |
Datasheet Extract
| Mouse spleen lysate (7 days old) | |
| Concentration | 2 mg/ml |
| Background | The mouse spleen tissue was collected from seven (7) day-old mice. The lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris -HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris -HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol. |
| Format | Purification: Whole Tissue Lysate Purity: Whole Tissue Lysate BufferSystem: Whole tissue lysate in 1 X SDS-PAGE sample loading buffer with 5% b-mercaptoethanol |
| Applications | WB |
