GTX27910 A-549 Lysate
100 µg / no delivery possible
Quick Overview
A-549 Lysate
Product Description
A-549 Lysate
Properties
| Catalog Number | GTX27910 |
| Quantity | 100 µg |
| Price | no delivery possible |
| Delivery | Europe only |
| Manufacturer | GeneTex Inc. |
| Datasheet |  http://www.genetex.com/co ... |
Datasheet Extract
| A-549 Lysate | |
| Concentration | 2 mg/ml |
| Background | A549 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetraacetic acid, 1 mM phenyl methyl sulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol. |
| Format | State: It is supplied as whole cell lysate, in 1 x SDS sample buffer containing 5% b-mercaptoethanol. Purification: Whole Cell Lysate Purity: Whole Cell Lysate BufferSystem: Whole lysate in 1 X SDS 5% b-mercaptoethanol |
| Applications | WB |
| Storage | Upon receipt - Store at -20 |
