NB100-105 Hypoxia-inducible factor 1 alpha antibody
see related secondary antibodiessee all 63 Hypoxia-inducible factor 1 alpha products
0.25 ml / US$ 435 no delivery possible
Quick Overview
Mouse anti Hypoxia-inducible factor 1 alpha H1alpha67
Synonyms
HIF1A, MOP1, MOP-1, HIF-1 alpha, HIF1 alpha, ARNT-interacting protein, Member of PAS protein 1, Basic-helix-loop-helix-PAS protein MOP1, Hypoxia-inducible factor 1 alpha,
Product Description
Mouse anti Hypoxia-inducible factor 1 alpha H1alpha67, Presentation: Purified. Product is tested for Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
Properties
| Applications | Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Monkey ( Mky ), Sheep ( Sh ), Mouse ( Ms ), Rat ( Rt ), Rabbit ( Rb ), Por ( Por ), Bovine ( Bov ), Ferret ( Ferret ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG2b |
| Clone | H1alpha67 |
| Catalog Number | NB100-105 |
| Swiss Prot. No. | Q16665 |
| Quantity | 0.25 ml |
| Price | US$ 435 no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Novus Biologicals Inc. |
| Datasheet | view  NB100-105.pdf |
Datasheet Extract
| Mouse Monoclonal anti-HIF-1 alpha (H1alpha67) | |
| Alternate names | anti-Hypoxia-inducible factor 1 alpha antibody, anti-HIF1 alpha antibody, anti-ARNT interacting protein antibody, anti-Hif1a antibody, anti-ARNT interacting protein antibody, anti-HIF-1alpha antibody, anti-Hypoxia inducible factor 1 alpha antibody, anti-Hypoxia inducible factor 1 alpha subunit basic helix antibody |
| Concentration | 4.0 mg/ml |
| Background | Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.
HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets. |
| Immunogen | Fusion protein containing amino acids 432-528 of human HIF-1alpha. Swiss Num.: Q16665 |
| Format | Purification: protein G purified Purity: protein G purified BufferSystem: PBS |
| Applications | chromatin immunoprecipitation , immunohistochemistry 1:25, immunofluorescence , immunoprecipitation Assay Dependent, Western Blot 1:500, Immunohistochemistry-Frozen 1:100 |
| Specificity | Species: NB 100-105 recognizes human, monkey, sheep, mouse, rat, rabbit, pig, bovine and ferret HIF-1alpha. |
| Storage | Store at 4C. Do not freeze. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'NB100-105 Hypoxia-inducible factor 1 alpha'.
