NB100-148 Rad51 antibody
see related secondary antibodiessee all 26 Rad51 products
0.1 mg / US$ 485 no delivery possible
Quick Overview
Mouse anti Rad51 14B4
Synonyms
hRAD51,
Product Description
Mouse anti Rad51 14B4, Presentation: Purified. Product is tested for Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
Properties
| Applications | Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG2b |
| Clone | 14B4 |
| Catalog Number | NB100-148 |
| Quantity | 0.1 mg |
| Price | US$ 485 no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Novus Biologicals Inc. |
| Datasheet |  http://www.novusbio.com/p ... |
Datasheet Extract
| Mouse Monoclonal anti-RAD51 (14B4) | |
| Alternate names | anti-DNA repair protein RAD51 homolog 1 antibody, anti-E coli RecA homolog antibody, anti-HGNC:9817 antibody, anti-Homolog of E coli RecA antibody, anti-homolog of S cerevisiae RAD51 antibody, anti-HRAD51 antibody, anti-HsRad51 antibody, anti-HsT16930 antibody, anti-RAD51 S cerevisiae homolog antibody, anti-RAD51A antibody, anti-RECA antibody, anti-RecA like protein antibody, anti-recombination protein A antibody |
| Concentration | 1 mg/ml |
| Background | Rad51, a 37kDa protein, is the human homolog of E. coli RecA protein and a member of the RAD52 epistasis group in S. cerevisiae. In recent studies it has been reported that BRCA1 interacts with Rad51 and disease-causing mutations have been found in the BRCA1 region necessary for BRCA1/Rad51 interaction, implying that this interaction is important for tumor suppression. BRCA2 and Rad51 have also been shown to interact by direct binding of the BRC repeats located within exon 11 of BRCA2. When these repeats are deleted, the interaction is lost and cells become hypersensitive to the DNA damage caused by methyl methanesulfonate (MMS).
|
| Immunogen | Fusion protein encoding full length (amino acids 1-338) of Rad51 expressed in E. coli. |
| Format | Purification: protein G purified Purity: protein G purified BufferSystem: PBS [pH7.4] |
| Applications | Western Blot, immunoprecipitation, immunofluorescence |
| Specificity | Species: NB 100-148 reacts with human Rad51. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| References | 1.Shinohara, A., Ogawa, H., Matsuda, Y., Ushio, N., Ikeo, K. and Ogawa, T. (1993) Cloning of human, mouse and fission yeast recombination genes homologous to RAD51 and recA. Nat Genet 4(3):239-43.
2. Scully, R., Chen , J., Plug, A., Xiao, Y., Weaver, D., Feunteun, J., Ashley, T. and Livingston, D.M. (1997) Association of BRCA1 with Rad51 in mitotic and meiotic cells. Cell 88:265-75. 3. Chen, P.L., et al. The BRC repeats in BRCA2 are critical for RAD51 binding and resistance to methyl methanesulfonate treatment. PNAS. 95:5287-5292, 1998. 1. Tarsounas, M., et al. BRCA2-dependent and independent formation of RAD51 nuclear foci. Oncogene. 22:1115-1123, 2001. 2. French, C.A., et al. Role of Mammalian RAD51L2 (RAD51C) in recombination and genetic stability. J. Biol. Chem. 277:19322-19330, 2002. 3. Masson, J-Y., et al. Identification and purification of two distinct complexes containing the five RAD51 paralogs. Genves Dev. 15:3296-3307, 2001. 4. Kao, G.D., et al. HIstone deacetylase 4 interacts with 53BPI to mediate the DNA damage response. J. Cell Biol. 160:1017-1027, 2003. 5. Masson, J-Y., et al. The meiosis-specific recombinase hDmc1 forms ring structures and interacts with hRad51, Embo. 18:-, 1999. 6. Warren, M., et al. Phenotypic effects of heterozygosity for a BRCA2 mutation. Hum. Mol. Genet. 12: 2645-2656, 2003. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'NB100-148 Rad51'.
