NB100-2220L LC3 antibody
see related secondary antibodiessee all 32 LC3 products
0.1 ml / US$ 465 no delivery possible
Quick Overview
Rabbit anti LC3
Synonyms
anti-Microtubule-associated proteins 1A/1B light chain 3B MAP1A/MAP1B LC3 B antibody; anti-MAP1A/b light chain 3 B antibody; anti-MAP1 light chain 3-like protein 2 antibody; anti-Autophagy-related protein LC3 B antibody; anti-Autophagy-related ubiquitin-like modifier LC3 B antibody,
Product Description
Rabbit anti LC3 , Presentation: Aff - Purified. Product is tested for Frozen sections ( C ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ), Paraffin Sections ( P )
Properties
| Applications | Frozen sections ( C ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ), Paraffin Sections ( P ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ), Rat ( Rt ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | NB100-2220L |
| Quantity | 0.1 ml |
| Price | US$ 465 no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Novus Biologicals Inc. |
| Datasheet |  http://www.novusbio.com/d ... |
Datasheet Extract
| Rabbit Polyclonal anti-LC3 , conjugated to HiLyte Flour? 488 | |
| Alternate names | anti-Microtubule-associated proteins 1A/1B light chain 3B MAP1A/MAP1B LC3 B antibody; anti-MAP1A/b light chain 3 B antibody; anti-MAP1 light chain 3-like protein 2 antibody; anti-Autophagy-related protein LC3 B antibody; anti-Autophagy-related ubiquitin-like modifier LC3 B antibody |
| Concentration | 1.06 mg/ml |
| Background | LC3, a mammalian homologue of Apg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. However, LC3 is now thought to also be involved in autophagy. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. LC3-II is the first mammalian protein identified that specifically associates with the autophagosome membranes. |
| Immunogen | A synthetic peptide made to an N-terminal portion of the human LC3 protein sequence (between residues 1-100). |
| Format | Purification: Immunogen affinity purified Purity: Immunogen affinity purified BufferSystem: Tris-glycine, 150mM NaCl |
| Applications | immunohistochemistry 1:200-1:400,,immunoprecipitation 20ug/500ug of protein,,Western Blot 2 ug/ml,,Immunohistochemistry-Paraffin ,, |
| Specificity | Species: This antibody reacts with human and mouse LC3 protein, though the mouse detection is weaker than the human. No other species have been tested. The immunogen used for this antibody production has 100% homology with rat and Zebrafish, 92% with mouse, 91% with cow, and 84% with Xenopus proteins. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| References | 1. Mizushima, N., et al. Dissection of autophagosome formation using Apg5- deficient mouse embryonic stem cells. J. Cell Biol. 152:657-668 (2001) |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'NB100-2220L LC3'.