NB100-2331L LC3 antibody
see related secondary antibodiessee all 35 LC3 products
0.1 ml / US$ 465 no delivery possible
Quick Overview
Rabbit anti LC3
Synonyms
anti-Microtubule-associated proteins 1A/1B light chain 3B MAP1A/MAP1B LC3 B antibody, anti-MAP1A/b light chain 3 B antibody, anti-Autophagy-related protein LC3 B antibody, anti-Autophagy-related ubiquitin-like modifier LC3 B antibody,
Product Description
Rabbit anti LC3 , Presentation: HiLyte Fluor 488. Product is tested for Frozen sections ( C ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ), Paraffin Sections ( P )
Properties
| Applications | Frozen sections ( C ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ), Paraffin Sections ( P ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ) |
| Presentation | HiLyte Fluor 488 |
| Host | Rabbit |
| Catalog Number | NB100-2331L |
| Quantity | 0.1 ml |
| Price | US$ 465 no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Novus Biologicals Inc. |
| Datasheet |  http://www.novusbio.com/d ... |
Datasheet Extract
| Rabbit Polyclonal anti-LC3 , conjugated to HiLyte Flour? 488 | |
| Alternate names | anti-Microtubule-associated proteins 1A/1B light chain 3B MAP1A/MAP1B LC3 B antibody, anti-MAP1A/b light chain 3 B antibody, anti-Autophagy-related protein LC3 B antibody, anti-Autophagy-related ubiquitin-like modifier LC3 B antibody |
| Concentration | 0.91 mg/ml |
| Background | Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in human. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells. |
| Immunogen | A synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 50-150). |
| Format | Purification: Affinity purified Purity: Affinity purified BufferSystem: Tris-glycine, 150mM NaCl |
| Applications | immunohistochemistry 1:200-1:400,,immunoprecipitation 20ug/500ug of protein,,Western Blot 2 ug/ml,,Immunohistochemistry-Paraffin ,, |
| Specificity | Species: Human and mouse. Other species have not been tested. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| References | 1. Kabeya, Y., et al. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. EMBO. 19(21): 5720-5728 (2000). 2. Mizushima, N., et al. Dissection of autophagosome formation using Apg5- deficient mouse embryonic stem cells. J. Cell. Biol. 152: 657-668 (2001). |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'NB100-2331L LC3'.
