NB100-663 EIF2A antibody
see related secondary antibodiessee all 27 EIF2A products
0.1 mg / US$ 505 no delivery possible
Quick Overview
Mouse anti EIF2A EIF2A
Synonyms
eIF-2A, eIF2-alpha, EIF2S1, , Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, CDA02, MST089, MSTP004, MSTP089
Product review
Please read our product review about EIF2A: Antibodies to eukaryotic Initiation Factors (eIF).
Product Description
Mouse anti EIF2A EIF2A, Presentation: Purified. Product is tested for Western blot / Immunoblot ( WB )
Properties
| Applications | Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ), Rat ( Rt ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | EIF2A |
| Catalog Number | NB100-663 |
| Swiss Prot. No. | P05198 |
| Quantity | 0.1 mg |
| Price | US$ 505 no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Novus Biologicals Inc. |
| Datasheet | view  NB100-663.pdf |
Datasheet Extract
| Mouse Monoclonal anti-eIF-2A (EIF2a) | |
| Alternate names | anti-Eukaryotic Translation Initiation Factor 2 alpha antibody, anti-CDA 02 antibody, anti-CDA02 antibody, anti-EIF-2alpha antibody, anti-eIF2S1 antibody |
| Concentration | 0.5 mg/ml |
| Background | eIF-2 alpha is a 36 kDa protein which is ubiquitously expressed in many cell types. The eIF-2 protein, which is composed of three subunits (alpha, beta and gamma), is one of the key molecules in the initiation of translation. In mammalian cells, eIF-2 alpha is phosphorylated at serine 52 by at least two kinases: the haem-controlled repressor (HCR) and the interferon inducible double stranded RNA-dependent protein kinase (PKR). Phosphorylation of eIF-2 alpha blocks the GDP-GTP exchange activity of eIF-2 beta, resulting in the suppression of protein synthesis. The phosphorylation of eIF-2 alpha is an important regulatory process in protein synthesis. |
| Immunogen | Recombinant full length protein (Human). Swiss Num.: P05198 |
| Format | Purification: protein A purified Purity: protein A purified BufferSystem: PBS, [pH 7.4] |
| Applications | Western Blot 1:500-1:1,000 |
| Specificity | Species: Human, Mouse, and Rat. Other species have not been tested. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| References | 1. Patel C.V., et al. PACT, a stress-modulated cellular activator of interferon-induced double-stranded RNA-activated protein kinase, PKR. J. Biol. Chem. 275: 37993-8 (2000). [PUBMED:10988289]
2. Datta B., et al. Increased phosphorylation of eukaryotic initiation factor 2alpha at the G2/M boundary in human osteosarcoma cells correlates with deglycosylation of p67 and a decreased rate of protein synthesis. Exp. Cell Res. 250: 223-30 (1999). [PUBMED:10388536] 3. Kimball S.R. Eukaryotic initiation factor eIF2. Int. J. Biochem. Cell Biol. 31: 25-9 (1999). [PUBMED:10216940] 4. Ernst H., et al. Cloning and sequencing of complementary DNAs encoding the alpha-subunit of translational initiation factor eIF-2. Characterization of the protein and its messenger RNA. J. Biol. Chem. 262: 1206-12 (1987). [PUBMED:2948954] 5. Lu, J., et al. The interferon-induced double-stranded RNA-activated protein kinase PKR will phosphorylate serine, threonine, or tyrosine at residue 51 in eukaryotic initiation factor 2alpha. J. Biol. Chem. 274(45): 32198-32203, (1999). 6. Kimball, S.R., et al. Purification and characterization of eukaryotic translational initiation factor eIF-2B from liver. Biochem. Biophys. Acta. 1201(3): 473-481, (1994). 7. Pavitt, G.D., et al. eIF2 independently binds two distinct eIF2B subcomplexes that catalyze and regulate guanine-nucleotide exchange. Genes Dev. 12(4): 514-526, (1999). 8. Zhang, P.C., et al. The PERK eukaryotic initiation factor 2 alpha kinase is required for the development of the skeletal system. postnatal growth, and the function and viability of the pancreas. Mol. Cell. Biol. 22(11): 3864-3874, (2002). 1. Ramos-Castaneda, J., et al. Deficiency of ATP2C1, a Golgi ion pump, induces secretory pathway defects in endoplasmic reticulum (ER)-associated degradation and sensitivity to ER stress. J. Biol. Chem. 280: 9467-73 (2005). [PubMed: 15623514] 2. Trottier, M.D., et al. VSV replication in neurons is inhibited by type I IFN at multiple stages of infection. Virology 333: 215-25 (2005). [PubMed: 15721356] |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'NB100-663 EIF2A'.
