NB100-75285 Early endosome antigen 1 antibody
see related secondary antibodiessee all 15 Early endosome antigen 1 products
0.05 mg / US$ 485 no delivery possible
Quick Overview
Chicken anti Early endosome antigen 1
Synonyms
anti-Early endosome antigen 1 antibody, anti-Early endosome associated protein antibody, anti-EEA 1 antibody, anti-EEA1 antibody, anti-Endosome associated protein p162 antibody, anti-ZFYVE2 antibody, anti-Zinc finger FYVE domain containing protein 2 antibody, Endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2
Product Description
Chicken anti Early endosome antigen 1 , Presentation: Aff - Purified. Product is tested for Immunofluorescence ( IF ), Western blot / Immunoblot ( WB )
Properties
| Applications | Immunofluorescence ( IF ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Aff - Purified |
| Host | Chicken |
| Catalog Number | NB100-75285 |
| Swiss Prot. No. | Q15075 |
| Quantity | 0.05 mg |
| Price | US$ 485 no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Novus Biologicals Inc. |
| Datasheet |  http://www.novusbio.com/d ... |
Datasheet Extract
| Chicken Polyclonal anti-EEA1 | |
| Alternate names | anti-Early endosome antigen 1 antibody, anti-Early endosome associated protein antibody, anti-EEA 1 antibody, anti-EEA1 antibody, anti-Endosome associated protein p162 antibody, anti-ZFYVE2 antibody, anti-Zinc finger FYVE domain containing protein 2 antibody |
| Background | Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc finger like domains, reminiscent of those found in nucleic acid binding proteins, are located in the amino and carboxyl terminal domains of EEA1. The carboxyl terminal zinc finger like domain is conserved in several other non nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown dependent on phosphatidyl 3 kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes.
Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking. |
| Immunogen | The immunogen for this antibody was a sequence from 307a.a. to 417a.a. from EEA1(a 1410a.a. long protein) Swiss Num.: Q15075 |
| Format | Purification: Immunogen affinity purified Purity: Immunogen affinity purified BufferSystem: Phosphate-Buffered Saline. |
| Applications | Immunocytochemistry, Western Blot |
| Specificity | Species: This antibody is reactive against Human. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| References | [1] Fratti,R.A., Chua,J. and Deretic,V., et al. Induction of p38 mitogen-activated protein kinase reduces early endosome autoantigen 1 (EEA1) recruitment to phagosomal membranes
[2] Dumas,J.J., Merithew,E., Sudharshan,E., Rajamani,D., Hayes,S., Lawe,D., Corvera,S. and Lambright,D.G. Multivalent endosome targeting by homodimeric EEA1 [3] Cheung,P.C., Trinkle-Mulcahy,L., Cohen,P. and Lucocq,J.M., et al. Characterization of a novel phosphatidylinositol 3-phosphate-binding protein containing two FYVE fingers in tandem that is targeted to the Golgi [4] Callaghan,J., Nixon,S., Bucci,C., Toh,B.H. and Stenmark,H., et al. Direct interaction of EEA1 with Rab5b [5] Simonsen,A., Lippe,R., Christoforidis,S., Gaullier,J.M., Brech,A., Callaghan,J., Toh,B.H., Murphy,C., Zerial,M. and Stenmark,H. EEA1 links PI(3)K function to Rab5 regulation of endosome fusion [6] , Mu F.-T., Callaghan J.M., Steele-Mortimer O., Stenmark H., Parton R.G., Campbell P.L., McCluskey J., Yeo J.-P., Tock E.P.C., Toh B.-H., et al. EEA1, an early endosome-associated protein. EEA1 is a conserved alpha-helical peripheral membrane protein flanked by cysteine 'fingers' and contains a calmodulin-binding IQ motif. [7] , Seelig H.P. Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases. [8] , Simonsen A., Lippe R., Christoforidis S., Gaullier J.-M., Brech A., Callaghan J.M., Toh B.-H., Murphy C., Zerial M., Stenmark H., et al. EEA1 links PI(3)K function to Rab5 regulation of endosome fusion. [9] , Callaghan J.M., Nixon S., Bucci C., Toh B.-H., Stenmark H. Direct interaction of EEA1 with Rab5b. [10] , Simonsen A., Gaullier J.-M., D'Arrigo A., Stenmark H. The Rab5 effector EEA1 interacts directly with syntaxin-6. |
11 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1300B | Chicken IgG (H&L) | ||||||
| Rabbit | Biotin | 1.5 mg / US$ 325 | |||||
| R1300F | Chicken IgG (H&L) | ||||||
| Rabbit | FITC | 1.5 mg / US$ 295 | |||||
| R1300T | Chicken IgG (H&L) | ||||||
| Rabbit | TRITC | 1.5 mg / US$ 305 | |||||
| R1300TR | Chicken IgG (H&L) | ||||||
| Rabbit | Texas Red | 1.5 mg / US$ 335 | |||||
| R1300HRP | Chicken IgG (H&L) | ||||||
| Rabbit | HRP | 1.5 mg / US$ 315 | |||||
| R1300AP | Chicken IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 355 | |||||
| R1434C2 | Chicken IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1434C3 | Chicken IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1434C35 | Chicken IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1434C5 | Chicken IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1434C55 | Chicken IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
Click here to see all secondary antibodies for 'NB100-75285 Early endosome antigen 1'.
