NB100-79944 FAK pTyr576/577 antibody
see related secondary antibodies
0.1 ml / US$ 535 no delivery possible
Quick Overview
Rabbit anti FAK pTyr576/577 EP1832Y
Synonyms
Protein-Tyrosine kinase, PTK2, FAK1, pp125FAK, FADK 1, , Focal Adhesion kinase 1, FAK-1, FADK1, FADK-1, Protein-tyrosine kinase 2
Product review
Please read our product review about FAK pTyr576/577: Antibodies to Focal Adhesion Kinase (FAK).
Product Description
Rabbit anti FAK pTyr576/577 EP1832Y. Product is tested for Western blot / Immunoblot ( WB ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Flow cytometry ( F )
Properties
| Applications | Western blot / Immunoblot ( WB ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Flow cytometry ( F ) |
| Reactivity | Human ( Hu ) |
| Host | Rabbit |
| Isotype | IgG |
| Clone | EP1832Y |
| Catalog Number | NB100-79944 |
| Swiss Prot. No. | Q05397 |
| Quantity | 0.1 ml |
| Price | US$ 535 no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Novus Biologicals Inc. |
| Datasheet |  http://www.novusbio.com/d ... |
Datasheet Extract
| Rabbit Monoclonal anti-FAK Phospho (EP1832Y) [Tyr576/577] | |
| Alternate names | Anti-FADK 1 antibody, Anti-pp125FAK antibody, Anti-Protein-tyrosine kinase 2 antibody |
| Background | Focal adhesion kinase (FAK) is a non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis (1). FAK is composed of a central catalytic domain flanked by large N- and C-terminal regions. FAK is activated by phosphorylation at tyrosine 397 in response to integrin clustering which can be induced by cell adhesion or antibody cross-linking or via G-protein-coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid (2-3). Phosphorylation of FAK Tyr-397 creates a binding site for Src-family kinases, and phosphorylation of FAK Tyr-576/Tyr-577 in the kinase domain activation loop enhances catalytic activity (4). Increased FAK expression has been correlated with the enhanced motility and invasiveness of human tumor cells, as well as with promoting increased cell proliferation (5). |
| Immunogen | A phospho-specific peptide corresponding to residues surrounding Tyrosine 576/577 of human FAK was used as an immunogen. This antibody detects FAK phosphorylated at Y576/577. Swiss Num.: Q05397 |
| Applications | Western Blot 1:50000-100000,Immunocytochemistry 1:50-100,immunoprecipitation 1:40,flow cytometry / FACS analysis 1:20 |
| Specificity | Species: This antibody is reactive against Human |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| References | 1. Schaller, M.D. (2001) Biochim. Biophys. Acta 1540, 1-21 2. Schaller, M.D., et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 5192-5196 3. Salazar, E.P, et al. (1999) J. Biol. Chem. 274, 28371-28378 4. Tremblay L., et al. Int. J. Cancer, 68: 164-171, 1996 |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'NB100-79944 FAK pTyr576/577'.
