NB100-80006 NEDD8 antibody
see related secondary antibodiessee all 9 NEDD8 products
0.1 ml / US$ 485 no delivery possible
Quick Overview
Rabbit anti NEDD8 Y297
Product Description
Rabbit anti NEDD8 Y297. Product is tested for Western blot / Immunoblot ( WB ), Frozen sections ( C ), Immunofluorescence ( IF ), Immunoprecipitation ( IP )
Properties
| Applications | Western blot / Immunoblot ( WB ), Frozen sections ( C ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ) |
| Reactivity | Human ( Hu ) |
| Host | Rabbit |
| Isotype | IgG |
| Clone | Y297 |
| Catalog Number | NB100-80006 |
| Quantity | 0.1 ml |
| Price | US$ 485 no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Novus Biologicals Inc. |
| Datasheet |  http://www.novusbio.com/d ... |
Datasheet Extract
| Rabbit Monoclonal anti-NEDD8 (Y297) | |
| Alternate names | Anti- antibody |
| Background | NEDD8 (Neural precursor cell-Expressed Developmentally Down-regulated genes) is an ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis (1). NEDD8 has been shown to conjugate to nuclear proteins in a manner analogous to ubiquination and sentrinization. The conjugation process is though to be catalyzed by thee enzymes; E1 (NEDD8-activation), E2 (NEDD8-conjugation) and E3 (NEDD8-ligation). NEDD8 is known to conjugate to Cul-4A and other cullins proteins via the carboxy-terminal Gly residue for ubiquitinylation (3) while NUB1 will negatively regulate its expression (4). |
| Immunogen | A synthetic peptide corresponding to residues in the N-term of human NEDD8 was used as immunogen. This antibody detects NEDD8 bound to cullins proteins (neddylated cullins) as well as free NEDD8. |
| Applications | Western Blot 1:500-1000,immunohistochemistry 1:100-250,Immunocytochemistry 1:100-250,immunoprecipitation 1:50 |
| Specificity | Species: This antibody is reactive against Human |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| References | 1. Kumar, S., et al. 1993. Biochem. Biophys. Res. Commun. 195: 393-399 2. Gong, L., et al. (1999) J. Biol. Chem. 274, 12036-12042 3. Kipreos, E.T., et al. 1996. Cell 85: 829-839 4. Katsumi Kito,et al. Biol. Chem., Vol. 276, Issue 23, 20603-20609, June 8, 2001 |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'NB100-80006 NEDD8'.
