NB100-80027 EIF2AK2 / PKR antibody
see related secondary antibodiessee all 21 EIF2AK2 / PKR products
0.1 ml / US$ 485 no delivery possible
Quick Overview
Rabbit anti EIF2AK2 / PKR Y117
Synonyms
Anti-Interferon-inducible RNA-dependent protein kinase antibody, Anti-Eukaryotic translation initiation factor 2-alpha kinase 2 antibody, Anti-eIF-2A protein kinase 2 antibody, Anti-Protein kinase RNA-activated antibody, Anti-PKR antibody, Anti-p68 kinase antibody, Anti-P1/eIF-2A protein kinase antibody, Interferon-induced, double-stranded RNA-activated protein kinase, Interferon-inducible RNA-dependent protein kinase, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2, Protein kinase RNA-activated, P1/eIF-2A protein kinase
Product Description
Rabbit anti EIF2AK2 / PKR Y117. Product is tested for Western blot / Immunoblot ( WB ), Frozen sections ( C ), Immunofluorescence ( IF ), Immunoprecipitation ( IP )
Properties
| Applications | Western blot / Immunoblot ( WB ), Frozen sections ( C ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ) |
| Reactivity | Human ( Hu ) |
| Host | Rabbit |
| Isotype | IgG |
| Clone | Y117 |
| Catalog Number | NB100-80027 |
| Swiss Prot. No. | P19525 |
| Quantity | 0.1 ml |
| Price | US$ 485 no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Novus Biologicals Inc. |
| Datasheet |  http://www.novusbio.com/d ... |
Datasheet Extract
| Rabbit Monoclonal anti-PKR (Y117) | |
| Alternate names | Anti-Interferon-inducible RNA-dependent protein kinase antibody, Anti-Eukaryotic translation initiation factor 2-alpha kinase 2 antibody, Anti-eIF-2A protein kinase 2 antibody, Anti-Protein kinase RNA-activated antibody, Anti-PKR antibody, Anti-p68 kinase antibody, Anti-P1/eIF-2A protein kinase antibody |
| Background | Protein kinase R (PKR) is a serine/threonine kinase that is induced by interferon and activated by double-stranded RNA. Upon activation, PKR is autophosphorylated at multiple sites and phosphorylates the a-subunit of the eukaryotic initiation factor-2 eIF2 (eIF2a), inhibiting protein synthesis (1,2). PKR controls the activation of several transcription factors (1) and mediates the effects of interferon via its capacity to inhibit protein synthesis (2). It has been shown that the autophosphorylation sites Thr-446 and Thr-451 in the activation loop of Human PKR are critical for its kinase activity (3). |
| Immunogen | A synthetic peptide corresponding to residues near C-terminus of human PKR was used as immunogen. The antibody does not cross-react with other GCN2 family members. Swiss Num.: P19525 |
| Applications | Western Blot 1:5000-10000,immunohistochemistry 1:100,Immunocytochemistry 1:250-500,immunoprecipitation 1:80 |
| Specificity | Species: This antibody is reactive against Human |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| References | 1. Williams, B.R. PKR; a sentinel kinase for cellular stress. Oncogene 18: 6112-6120 (1999). 2. Cuddihy, A.R., et al. The double-stranded RNA activated protein kinase PKR physically associates with the tumor suppressor p53 protein and phosphorylates human p53 on serine 392 in vitro. Oncogene 18:2690-2702 (1999). 3. Zhang, F., et al. Binding of double-stranded RNA to protein kinase PKR is required for dimerization and promotes critical autophosphorylation events in the activation loop. J. Biol. Chem. 276: 24946-24958 (2001). |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'NB100-80027 EIF2AK2 / PKR '.
