NB100-80067 65 kDa Yes-associated protein antibody
see related secondary antibodiessee all 9 65 kDa Yes-associated protein products
0.1 ml / no delivery possible
Quick Overview
Rabbit anti 65 kDa Yes-associated protein EP1674Y
Synonyms
Anti-YAP65 antibody,
Product Description
Rabbit anti 65 kDa Yes-associated protein EP1674Y. Product is tested for Western blot / Immunoblot ( WB ), Frozen sections ( C ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Flow cytometry ( F )
Properties
| Applications | Western blot / Immunoblot ( WB ), Frozen sections ( C ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Flow cytometry ( F ) |
| Reactivity | Human ( Hu ) |
| Host | Rabbit |
| Isotype | IgG |
| Clone | EP1674Y |
| Catalog Number | NB100-80067 |
| Swiss Prot. No. | P46937 |
| Quantity | 0.1 ml |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Novus Biologicals Inc. |
| Datasheet |  http://www.novusbio.com/d ... |
Datasheet Extract
| Rabbit Monoclonal anti-YAP65 (EP1674Y) | |
| Alternate names | Anti-YAP65 antibody |
| Background | The transcriptional coactivator Yes-associated protein (YAP) has been shown to interact with and to enhance p73-dependent apoptosis in response to DNA damage. It has been shown that YAP requires the promyelocytic leukemia gene (PML) and nuclear body localization to coactivate p73. YAP imparts selectivity to p73 by promoting the activation of a subset of p53 and/or p73 target promoters (1). Akt promotes YAP localization to the cytoplasm, resulting in loss from the nucleus where it functions as a coactivator of transcription factors including p73. p73-mediated induction of Bax expression following DNA damage requires YAP function and is attenuated by Akt phosphorylation of YAP. YAP overexpression increases, while YAP depletion decreases, p73-mediated apoptosis following DNA damage, in an Akt inhibitable manner (2) |
| Immunogen | A synthetic peptide corresponding to residues near the C-terminus of human YAP65 was used as an immunogen. Swiss Num.: P46937 |
| Applications | Western Blot 1:25000-50000,immunohistochemistry 1:100-250,Immunocytochemistry 1:100-250,immunoprecipitation 1:70,flow cytometry / FACS analysis 1:25 |
| Specificity | Species: This antibody is reactive against Human |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| References | 1. Strano S, et al. Mol Cell 18(4):447-59, 2005 2. Basu S, et al. Mol Cell 11(1):11-23, 2003 |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'NB100-80067 65 kDa Yes-associated protein'.