NB100-80068 65 kDa Yes-associated protein antibody
see related secondary antibodiessee all 9 65 kDa Yes-associated protein products
0.1 ml / no delivery possible
Quick Overview
Rabbit anti 65 kDa Yes-associated protein EP1675Y
Synonyms
YAP1 YAP65
Product Description
Rabbit anti 65 kDa Yes-associated protein EP1675Y. Product is tested for Westernblot / Immunoblot ( WB ), Frozen sections ( C )
Properties
| Applications | Westernblot / Immunoblot ( WB ), Frozen sections ( C ) |
| Reactivity | Human ( Hu ) |
| Host | Rabbit |
| Isotype | IgG |
| Clone | EP1675Y |
| Catalog Number | NB100-80068 |
| Swiss Prot. No. | P46937 |
| Quantity | 0.1 ml |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Novus Biologicals Inc. |
| Datasheet |  http://www.novusbio.com/d ... |
Datasheet Extract
| Rabbit Monoclonal anti-YAP65 Phospho (EP1675Y) [Ser127] | |
| Alternate names | Anti-YAP65 antibody |
| Background | The transcriptional coactivator Yes-associated protein (YAP, YAP65) has been shown to interact with and to enhance p73-dependent apoptosis in response to DNA damage. It has been shown that YAP requires the promyelocytic leukemia gene (PML) and nuclear body localization to coactivate p73. YAP imparts selectivity to p73 by promoting the activation of a subset of p53 and/or p73 target promoters (1). YAP binds with 14-3-3 after being phosphorylated by Akt at Ser127. Akt promotes YAP localization to the cytoplasm, resulting in loss from the nucleus where it functions as a coactivator of transcription factors including p73. p73-mediated induction of Bax expression following DNA damage requires YAP function and is attenuated by Akt phosphorylation of YAP. YAP overexpression increases, while YAP depletion decreases, p73-mediated apoptosis following DNA damage, in an Akt inhibitable manner (2) |
| Immunogen | A phospho specific peptide corresponding to residues surrounding Ser127 of human YAP65 was used as immunogen. The antibody detects YAP65 phosphorylated at Serine 127. Swiss Num.: P46937 |
| Applications | Western Blot 1:10000-50000,immunohistochemistry 1:100-250 |
| Specificity | Species: This antibody is reactive against Human |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| References | 1. Strano S, et al. Mol Cell 18(4):447-59, 2005 2. Basu S, et al. Mol Cell 11(1):11-23, 20033. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / 170,00 € | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / 160,00 € | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / 160,00 € | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / 170,00 € | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / 170,00 € | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / 200,00 € | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / 320,00 € | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / 320,00 € | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / 320,00 € | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / 320,00 € | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / 320,00 € | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 330,00 € | |||||
Click here to see all secondary antibodies for 'NB100-80068 65 kDa Yes-associated protein'.
