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NB120-16048  Lamin B1 antibody

see related secondary antibodies
see all 8 Lamin B1 products
0.1 mg / US$ 565 no delivery possible
please contact the manufacturer
Novus Biologicals
Littleton, CO, USA
www.novus-biologicals.com

Quick Overview

Rabbit anti Lamin B1

Product Description

Rabbit anti Lamin B1 , Presentation: Aff - Purified. Product is tested for Western blot / Immunoblot ( WB )

Properties

ApplicationsWestern blot / Immunoblot ( WB )
PresentationAff - Purified
HostRabbit
IsotypeIgG
Catalog NumberNB120-16048
Quantity0.1 mg
PriceUS$ 565 no delivery possible
DeliveryNot USA/Canada
ManufacturerNovus Biologicals Inc.
DatasheetPDF-Download
http://www.novusbio.com/d ...

Datasheet Extract

Rabbit Polyclonal anti-Lamin B1
Concentration0.1 mg/ml
BackgroundLamin B1 and Lamin B antibodies are extremely useful as nuclear loading controls for use with nuclear extracts. When using Lamin B1 antibodies as nuclear loading controls, be aware that in apoptotic cells Lamin B1 is cleaved (Kottke TJ et al.). Lamin B1 will also be removed from a nuclear prep if the nuclear membranes are spun out. This antibody was designed to be a nuclear loading control however it has not yet been tested in appropriate lysates.
ImmunogenSynthetic peptide derived from residues 400 - 500 of Mouse Lamin B1.(Note: the amino acid sequence is proprietary).
Format
Purification: Immunogen affinity purified
Purity: Immunogen affinity purified
BufferSystem: Preservative: 0.02% Sodium AzideConstituents: 1% BSA, PBS. pH 7.4
ApplicationsWB
Specificity
Species: Cross-reacts with Human.Expected to cross-react with Mouse (100% identity with immunogen), Rat (100% identity with immunogen), Chicken (99% identity with immunogen), Xenopus laevis (92% identity with immunogen) and Zebrafish (100% identity with immunogen) due to sequence homology.Not yet tested in other species.
ReferencesGeneral / background references:Bosco EE & Knudsen ES Differential role of RB in response to UV and IR damage. Nucleic Acids Res 33:1581-92 (2005). Wong K et al. Nerve growth factor receptor signaling induces histone acetyltransferase domain-dependent nuclear translocation of p300/CREB-binding protein-associated factor and hGCN5 acetyltransferases. J Biol Chem 279:55667-74 (2004). Dunaeva M et al. Characterization of the physical interaction of Gli proteins with SUFU proteins. J Biol Chem 278:5116-22 (2003). Pardo OE et al. Fibroblast growth factor 2-mediated translational control of IAPs blocks mitochondrial release of Smac/DIABLO and apoptosis in small cell lung cancer cells. Mol Cell Biol 23:7600-10 (2003). Feng B et al. The endoplasmic reticulum is the site of cholesterol-induced cytotoxicity in macrophages. Nat Cell Biol 5:781-92 (2003). Kottke TJ et al. Comparison of paclitaxel-, 5-fluoro-2'-deoxyuridine-, and epidermal growth factor (EGF)-induced apoptosis. Evidence for EGF-induced anoikis. J Biol Chem 274:15927-36 (1999).

12 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1364B Rabbit IgG (H&L)  
  Goat Biotin   2 mg / US$ 295
   
R1364F Rabbit IgG (H&L)  
  Goat FITC   2 mg / US$ 285
   
R1364T Rabbit IgG (H&L)  
  Goat TRITC   2 mg / US$ 285
   
R1364TR Rabbit IgG (H&L)  
  Goat Texas Red   2 mg / US$ 295
   
R1364HRP Rabbit IgG (H&L)  
  Goat HRP   2 mg / US$ 295
   
R1364AP Rabbit IgG (H&L)  
  Goat AP   1 mg / US$ 325
   
R1458C2 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / US$ 445
   
R1458C3 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / US$ 445
   
R1458C35 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / US$ 445
   
R1458C5 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / US$ 445
   
R1458C55 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / US$ 445
   
R1427R Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 455
   

Click here to see all secondary antibodies for 'NB120-16048 Lamin B1'.