NB300-209 Dynamin (pSer774) antibody
see related secondary antibodiessee all 48 Dynamin products
0.1 ml / US$ 485 no delivery possible
Quick Overview
Sheep anti Dynamin (pSer774)
Synonyms
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Product review
Please read our product review about Dynamin (pSer774): Antibodies to Dynamins.
Product Description
Sheep anti Dynamin (pSer774) , Presentation: Aff - Purified. Product is tested for Frozen sections ( C ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ), Rat ( Rt ) |
| Presentation | Aff - Purified |
| Host | Sheep |
| Catalog Number | NB300-209 |
| Quantity | 0.1 ml |
| Price | US$ 485 no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Novus Biologicals Inc. |
| Datasheet |  http://www.novusbio.com/p ... |
Datasheet Extract
| Sheep Polyclonal anti-Dynamin [Ser774] | |
| Alternate names | Anti-DNM 1 antibody; Anti-DNM antibody; Anti-DNM1 antibody; Anti-Dynamin 1 antibody; Anti-Dynamin1 antibody; Anti-Neuronal dynamin antibody |
| Background | Dynamins are a family of GTPases transcribed from at least three separate genes. They contain several conserved regions; an amino-terminal GTPase domain, a centrally located membrane-binding plekstrin homology domain (PHD), and a region located in front of the proline-rich domain (PRD). This PRD region is thought to mediate interactions between dynamin and other cellular proteins. There are also at least three isoforms of this protein. Dynamin 2 is ubiquitous. Dynamin 3 is expressed in the brain, testis, heart and lung. Dynamin 1 is exclusively neuronal and is phosphorylated by PKC and dephosphorylated by calcineurin during an action potential in the nerve terminal. It is believed that this dephosphorylation provides a trigger for the cellular process of clatherin-mediated and fluid-phase endocytosis. |
| Immunogen | NB 300-209 was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated Ser774 of rat Dynamin. This is the site phosphorylated by cyclin dependent protein kinase 5 (CDK5). The antigen is identical in human, rat, mouse. |
| Format | Purification: Affinity purified Purity: Affinity purified BufferSystem: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. |
| Applications | Western Blot 1:1,000 |
| Specificity | Species: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse and non-human primates based on the fact that these species have 100% homology with the amino acid sequence used as antigen. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| References | 1. Tan, TC, et al. Cdk5 is Essential for Synaptic Vesicle Endocytosis. Nat. Cell Bio., 5(8): 701-710. 2003 |
7 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1456B | Sheep IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 295 | |||||
| R1456F | Sheep IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1456T | Sheep IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1456TR | Sheep IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1456HRP | Sheep IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1456AP | Sheep IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1433R | Sheep IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'NB300-209 Dynamin (pSer774)'.
