NB300-306 Raf-1 pSer642 antibody
see related secondary antibodies
0.1 ml / US$ 455 no delivery possible
Quick Overview
Rabbit anti Raf-1 pSer642
Synonyms
Raf1,
Product Description
Rabbit anti Raf-1 pSer642 , Presentation: Aff - Purified. Product is tested for Western blot / Immunoblot ( WB )
Properties
| Applications | Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ), Rat ( Rt ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | NB300-306 |
| Quantity | 0.1 ml |
| Price | US$ 455 no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Novus Biologicals Inc. |
| Datasheet | view  NB300-306.pdf |
Datasheet Extract
| Rabbit Polyclonal anti-RAF1 [Ser642] | |
| Alternate names | anti-cRaf1 transforming gene antibody, anti-Murine sarcoma 3611 oncogene 1 antibody, anti-Oncogene RAF1 antibody, anti-Raf proto oncogene serine threonine protein kinase antibody, anti-Sarcoma 3611 oncogene antibody, anti-vRaf1 murine leukemia viral oncogene homologue 1 antibody |
| Background | The Ras pathway is a critical signal transduction cascade involved in regulating cellular proliferation, differentiation, survival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases MEK and ERK. Previous studies have shown that phosphorylation is required for Raf-1 activation. Recent work has demonstrated that phosphorylation also regulates the downregulation of Raf with two sites participating Ser 301 and Ser 642. |
| Immunogen | Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser642 of rat Raf-1. |
| Format | Purification: Immunogen affinity purified Purity: Immunogen affinity purified BufferSystem: 10 mM Hepes (pH7.5), 150 mM NaCl, 100 ug/ml BSA and 50% glycerol |
| Applications | Western Blot 1:1000 |
| Specificity | Species: Human, mouse, and rat based on 100% antigen sequence homology. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| References | 1. Chong H, Vikis HG, Guan KL (2003) Mechanisms of regulating the Raf kinase family. Cellular Signalling 15:463-469.
2. Dhillon AS, Kolch W (2002) Untying the regulation of the Raf-1 kinase. Arch Biochem Biophys 404:3-9. 3. Dougherty MK, Muller J, Ritt DA, Zhou M, Zhou XZ, Copeland TD, Conrads TP, Veenstra TD, Lu KP, Morrison DK (2005) Regulation of Raf-1 by Direct Feedback Phosphorylation. Mol Cell 17:215-224. 4. Marshall CJ (1996) Ras effectors. current opinion in cell biology 8:197-204. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'NB300-306 Raf-1 pSer642'.
