PA047 BDNF antigen
see all 35 BDNF products
2 µg / US$ 255
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Human BDNF
Synonyms
brain-derived Neurotrophic Factor, Abrineurin, MGC34632,
Product Description
Human BDNF
Properties
| Species | Human |
| Purity | >96% |
| Source | E. coli |
| Molecular Weight | 27 kD |
| Catalog Number | PA047 |
| Swiss Prot. No. | P23560 |
| Quantity | 2 µg |
| Price | US$ 255 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  PA047.pdf |
Datasheet Extract
| Background | BDNF is a member of the NGF family of neurotrophic growth factors. Like other members of this family, BDNF supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR, which also binds other neurotrophins such as NGF, NT-3 and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB. BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residue and a propeptide of 110 amino acid residues. |
| Immunogen | Swiss Num.: P23560 |
| Source | E. coli |
| Format | State: Lyophilized Purity: > 98% by SDS-PAGE gel and HPLC analyses Endotoxinlevel: < 0.1 ng per µg (1EU/µg) Reconstitution: Centrifuge the vial prior to opening! Restore in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 2-8°C for 1 week or -20°C for future use. |
| Description | Recombinant human BDNF is a homodimer of two 119 amino acid subunits linked by strong non-covalent interactions.
Human and Mouse BDNF sequences are identical. AASequence: MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR Biological Activity: Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 0.5 - 1.0 µg/ml. Molecular weight: 27.0 kDa |
| Storage | The lyophilized protein is stable at -20°C for one year from date of despatch.
Reconstituted human BDNF is stable for 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freezing and thawing. |
| References | 1. Merdek, K.D. Intrinsic signaling functions of the beta4 integrin intracellular domain. The Journal of Biological Chemistry.;282(41):30322-30. (2007)
2. Inoue, K. The Transcription Factor Runx3 Represses the Neurotrophin Receptor TrkB during Lineage Commitment of Dorsal Root Ganglion Neurons. Journal of Biological Chemistry.;282(33):24175-84. (2007) 3. Rao, M.S. Strategies for promoting anti-seizure effects of hippocampal fetal cells grafted into the hippocampus of rats exhibiting chronic temporal lobe epilepsy. Neurobiology of Disease.;27(2):117-32. (2007) 4. Geiger, T.R. Critical role for TrkB kinase function in anoikis suppression, tumorigenesis, and metastasis. Cancer Research.;67(13):6221-9 (2007) 5. Trajkovska, V. Measurements of brain-derived neurotrophic factor: methodological aspects and demographical data. Brain Research Bulletin.;73(1-3):143-9 (2007). 6. Rivera, C. J. of Cell Bio. Vol:159 (5) pp. 747-752 (2002). Haapasalo, A. J. Biol. Chem. Vol: 277 (45) pp. 43160-43167 (2002). 7. Diaz, E. Neuron Vol: 36 pp. 417-434 (2002). 8. Lee, FS. J. Bio. Chem. Vol: 277 (11) pp. 9096-9102 (2002). 9. Francis, N. J. Bio. Chem. Vol: 277 (8) pp. 6511-6519 (2002). 10. Q, L. Cell. Vol 105: pp.69-79 (2001). Atwal, JK. Neuron, Volume 27. Pp.265-277 (2000). 11. Salehi, AH. Neuron, Volume 27, pp.279-288 (2000). 12. Vicario-Abejon, C. The Journal of Neuroscience 18(18): pp 7256-7271 (1998). 13. Hanson, MG. The J. of Neuroscience Vol: 18(18): pp.7361-7371 (1998). 14. Struder, L. nature neuroscience . Volume 1 - no. 4 (1998). 15. Qiao, X. The Journal of Neuroscience 18(17):p 6990-6999 (1998). 16. Courtney, MJ. Journal of Physiology Volume 17. Number 11.pp.4201-4211 (1997). 17. Liou, JC. Journal of Physiology. 503.1. Pp.129-139 (1997). 18. Cheng, L. Neuron. Volume 16. Pp. 309-319 By Cell Press (1996). |
