PA094 Leptin antigen
see all 161 Leptin products
0.2 mg / US$ 255
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Human Leptin
Synonyms
Obese protein,
Product Description
Human Leptin
Properties
| Species | Human |
| Purity | >95% |
| Source | E. coli |
| Molecular Weight | 16 kD |
| Catalog Number | PA094 |
| Quantity | 0.2 mg |
| Price | US$ 255 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  PA094.pdf |
Datasheet Extract
| Alternate names | Obesity protein (OB)
|
| Background | Encoded by the ob (obese) gene, Leptin is an adipose-derived cytokine that suppresses appetite and increases thermogenesis. Leptin exerts its anorectic effect via signaling through a hypothalamic receptor termed OB-R. Leptin has been shown to reduce body weight, food consumption, and plasma glucose levels in various in vivo models. |
| Source | E.coli |
| Format | State: Lyophilized purified fraction (>98% pure by SDS-PAGE and HPLC analyses). BufferSystem: 0.1% TFA without preservatives. Endotoxinlevel: < 0.1 ng per µg (1EU/µg) Reconstitution: Restore in water to a concentration of 0.1-0.5 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 2-8°C for 1 week or -20°C for future use. |
| Description | Human Leptin is a protein containing 147 amino acid residues. AASequence: MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC Biological Activity: Human Leptin is biologically active in the ob/ob mouse obesity model. The ob/ob mice were treated via intraperitoneal injection once daily at a dose of 5 µg Leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls. Molecular weight: 16 kDa |
| Add. Information | Centrifuge the vial prior to opening! |
| Storage | Store the lyophilized protein at -20°C.
Reconstituted Leptin should be stored in working aliquots with a carrier protein at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Chang YJ, Disparate mesenchyme-lineage tendencies in mesenchymal stem cells from human bone marrow and umbilical cord blood Stem Cells, 2006, 24(3):679-85.
2. Ladenheim EE Leptin enhances feeding suppression and neural activation produced by systemically administered bombesin. Am J Physiol Regul Integr Comp Physiol., 2005, 289(2):R473-R477. 3. Bruce CR Cytokine regulation of skeletal muscle fatty acid metabolism: effect of interleukin-6 and tumor necrosis factor-alpha. Am J Physiol Endocrinol Metab., 2004, 287(4):E616-21. 4. Blevins JE Evidence that paraventricular nucleus oxytocin neurons link hypothalamic leptin action to caudal brain stem nuclei controlling meal size. Am J Physiol Regul Integr Comp Physiol., 2004, 287(1):R87-96. 5. Kathrin Maedler Leptin modulates beta cell expression of IL-1 receptor antagonist and release of IL-1beta in human islets PNAS, 2004, Vol: 101 (21) pp. 8138-8143. 6. Ruffin MP Feeding and temperature responses to intravenous leptin infusion are differential predictors of obesity in rats Am J Physiol Regul Integr Comp Physiol., 2004, 286(4):R756-63. |
