PA1006 Bone Morphogenetic Protein 7 (BMP7) antigen
see all 15 Bone Morphogenetic Protein 7 products
2 µg / US$ 255
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Human Bone Morphogenetic Protein 7 (BMP7)
Synonyms
BMP-7,
Product Description
Human Bone Morphogenetic Protein 7 (BMP7)
Properties
| Presentation | Purified |
| Species | Human |
| Purity | >98% |
| Source | CHO cells |
| Molecular Weight | 28.8 kD |
| Catalog Number | PA1006 |
| Quantity | 2 µg |
| Price | US$ 255 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  PA1006.pdf |
Datasheet Extract
| Alternate names | Osteogenic Protein 1, OP-1, BMP-7. |
| Concentration | 1.0 mg/ml |
| Background | BMP-7 (osteogenic protein 1) belongs to the Bone-growth regulatory factors that are members of the transforming growth factor-beta (TGF-beta) superfamily of proteins. They are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins. |
| Source | Escherichia Coli. |
| Format | State: Sterile Filtered White lyophilized (freeze-dried) powder. Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. BufferSystem: The protein was lyophilized from a concentrated sterile solution containing 10 mM sodium citrate pH 3.5. Endotoxinlevel: Less than 0.1 ng/µg (IEU/µg) of BMP-7. Dimers: Less than 1% as determined by silver-stained SDS-PAGE gel analysis. Reconstitution: It is recommended to reconstitute the lyophilized Bone Morphogenetic Protein-7 in sterile 20 mM AcOH (acetic Acid) not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
| Applications | Molecular standard (Western, ELISA) in studying secreted BMP-7.
Preparing antibodies for BMP-7 monomer. Molecule standard in detecting secreted BMP-7 in reduced SDS-PAGE. |
| Description | Recombinant Human BMP-7 produced in E.Coli is a monomeric, non-glycosylated, Polypeptide chain containing 139 amino acids.
The rHuBMP-7 is purified by proprietary chromatographic techniques. AASequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Thr-Gly-Ser-Lys. Molecular weight: 15679.97 Dalton. |
| Add. Information | Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a standard solution of BMP-7 as a Reference Standard. |
| Storage | Lyophilized rHuBMP-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuBMP-7 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freezing and thawing. Shelf life: one year from despatch |
| References | 1. Bone morphogenetic protein-7 is an antagonist of transforming growth factor-beta2 in human trabecular meshwork cells. Invest Ophthalmol Vis Sci 2007 Feb;48(2):715-26.
2. Herpes simplex virus type-1 latency-associated transcript-induced immunoreactivity of substance P in trigeminal neurons is reversed by bone morphogenetic protein-7. Neurosci Lett 2007 Feb 8;413(1):31-5. 3. Increased accumulation of superficial zone protein (SZP) in articular cartilage in response to bone morphogenetic protein-7 and growth factors. J Orthop Res 2007 Mar;25(3):293-303. 4. Bone morphogenetic protein 7 (bmp-7) stimulates proteoglycan synthesis in human osteoarthritic chondrocytes in vitro. Biomed Pharmacother 2006 Dec;60(10):639-43. 5. Renal bone morphogenetic protein-7 protects against diabetic nephropathy. J Am Soc Nephrol 2006 Sep;17(9):2504-12. 6. Function and effect of bone morphogenetic protein-7 in kidney bone and the bone-vascular links in chronic kidney disease. Eur J Clin Invest 2006 Aug;36 Suppl 2:43-50. |
