PA1007 Nerve Growth Factor beta (NGF beta) antigen
see all 12 Nerve Growth Factor beta products
5 µg / US$ 225
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Human Nerve Growth Factor beta (NGF beta)
Product Description
Human Nerve Growth Factor beta (NGF beta)
Properties
| Presentation | Purified |
| Species | Human |
| Purity | >98% |
| Source | CHO cells |
| Molecular Weight | 17 kD |
| Catalog Number | PA1007 |
| Quantity | 5 µg |
| Price | US$ 225 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  PA1007.pdf |
Datasheet Extract
| Alternate names | Beta Polypeptide, NGF, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428 |
| Background | NGF-beta has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. |
| Source | Chinese Hamster Ovary Cells |
| Format | State: Lyophilized purified protein (sterile filtered 0.2µm) Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. BufferSystem: 30mM sodium acetate pH=5.5 containing 1% HSA Reconstitution: Restore in sterile 18Mohm-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Description | Nerve Growth Factor-beta Human Recombinant produced in CHO is a homodimer, glycosylated, polypeptide chain containing 2 identical 119 amino acids. The NGF-b is purified by proprietary chromatographic techniques. AASequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ser-Ser-His-Pro. Biological Activity: The ED50, calculated by its ability to stimulate chick E9 DRG neurite outgrowth was found to be < 1.0 ng/ml. Specific Activity: > 1 x 106 units/mg Molecular weight: 16,950 Dalton |
| Add. Information | Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.1945 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Nerve Growth Factor-b Recombinant as a Reference Standard. |
| Storage | Prior to reconstitution stable at RT for 3 weeks.
Following reconstitution store the protein undiluted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Shelf life: one year from despatch. |
